BMRB Entry 26866

Name: SNX27 PDZ domain
Published: 2016-08-30

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26866

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SNX27 PDZ domain

Deposition date:

2016-08-04

Original release date:

2016-08-30

Authors:

Collins, Brett; Mas, Caroline; Clairfeuille, Thomas

Citation:

Citation: Clairfeuille, Thomas; Mas, Caroline; Chan, Audrey; Yang, Zhe; Tello-Lafoz, Maria; Chandra, Mintu; Widagdo, Jocelyn; Kerr, Markus; Paul, Blessy; Merida, Isabel; Teasdale, Rohan; Pavlos, Nathan; Anggono, Victor; Collins, Brett. "A molecular code for endosomal recycling of phosphorylated cargos by the SNX27-retromer complex" Nat. Struct. Mol. Biol. 23, 921-932 (2016).
PubMed: 27595347

Assembly members:

Assembly members:
PDZ_domain, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX4T-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDZ_domain: GSHGGSPRVVRIVKSESGYG FNVRGQVSEGGQLRSINGEL YAPLQHVSAVLPGGAADRAG VRKGDRILEVNGVNVEGATH KQVVDLIRAGEKELILTVLS V

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	276
15N chemical shifts	96
1H chemical shifts	96

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PDZ domain	1

Entities:

Entity 1, PDZ domain 101 residues - Formula weight is not available

1

GLY

SER

HIS

GLY

SER

PRO

ARG

VAL

2

ARG

ILE

VAL

LYS

SER

GLU

SER

GLY

TYR

GLY

3

PHE

ASN

VAL

ARG

GLY

GLN

VAL

SER

GLU

GLY

4

GLY

GLN

LEU

ARG

SER

ILE

ASN

GLY

GLU

LEU

5

TYR

ALA

PRO

LEU

GLN

HIS

VAL

SER

ALA

VAL

6

LEU

PRO

GLY

ALA

ASP

ARG

ALA

GLY

7

VAL

ARG

LYS

GLY

ASP

ARG

ILE

LEU

GLU

VAL

8

ASN

GLY

VAL

ASN

VAL

GLU

GLY

ALA

THR

HIS

9

LYS

GLN

VAL

ASP

LEU

ILE

ARG

ALA

GLY

10

GLU

LYS

GLU

LEU

ILE

LEU

THR

VAL

LEU

SER

11

VAL

Samples:

sample_1: PDZ domain, [U-100% 13C; U-100% 15N], 0.8 mM; Hepes 20 mM; NaCl 200 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker AMX 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks