BMRB Entry 26863
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26863
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NMR-STAR v3 text file.
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Citation: Zheng, Weiyue; Umitsu, Masataka; Jagan, Ishaan; Tran, Charles; Ishiyama, Noboru; BeGora, Michael; Araki, Kiyomi; Ohashi, Pamela; Ikura, Mitsuhiko; Muthuswamy, Senthil. "An interaction between Scribble and the NADPH oxidase complex controls M1 macrophage polarization and function" Nat. Cell. Biol. 18, 1244-1252 (2016).
PubMed: 27694890
Assembly members:
ScribPDZ4apo, polymer, 102 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
ScribPDZ4apo: GSHMPGLRELCIQKAPGERL
GISIRGGARGHAGNPRDPTD
EGIFISKVSPTGAAGRDGRL
RVGLRLLEVNQQSLLGLTHG
EAVQLLRSVGDTLTVLVCDG
FE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 176 |
| 15N chemical shifts | 82 |
| 1H chemical shifts | 82 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ScribPDZ4apo | 1 |
Entities:
Entity 1, ScribPDZ4apo 102 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | PRO | GLY | LEU | ARG | GLU | LEU | ||||
| 2 | CYS | ILE | GLN | LYS | ALA | PRO | GLY | GLU | ARG | LEU | ||||
| 3 | GLY | ILE | SER | ILE | ARG | GLY | GLY | ALA | ARG | GLY | ||||
| 4 | HIS | ALA | GLY | ASN | PRO | ARG | ASP | PRO | THR | ASP | ||||
| 5 | GLU | GLY | ILE | PHE | ILE | SER | LYS | VAL | SER | PRO | ||||
| 6 | THR | GLY | ALA | ALA | GLY | ARG | ASP | GLY | ARG | LEU | ||||
| 7 | ARG | VAL | GLY | LEU | ARG | LEU | LEU | GLU | VAL | ASN | ||||
| 8 | GLN | GLN | SER | LEU | LEU | GLY | LEU | THR | HIS | GLY | ||||
| 9 | GLU | ALA | VAL | GLN | LEU | LEU | ARG | SER | VAL | GLY | ||||
| 10 | ASP | THR | LEU | THR | VAL | LEU | VAL | CYS | ASP | GLY | ||||
| 11 | PHE | GLU |
Samples:
sample_1_15N: ScribPDZ4apo, [U-100% 15N], 0.2 mM; NaCl 100 mM
sample_2_15N13C: ScribPDZ4apo, [U-100% 13C; U-100% 15N], 0.1 mM; NaCl 100 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1_15N | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2_15N13C | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_2_15N13C | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_2_15N13C | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks