BMRB Entry 26862

Name: Stress Response Peptide-2; Manduca Sexta
Published: 2017-02-16

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26862

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Stress Response Peptide-2; Manduca Sexta

Deposition date:

2016-07-27

Original release date:

2017-02-16

Authors:

Schrag, Lynn; Cao, Xiaolong; Herrera, Alvaro; Wang, Yang; Jiang, Haobo; Prakash, Om

Citation:

Citation: Schrag, Lynn Goss; Cao, Xiaolong; Herrera, Alvaro Ivan; Wang, Yang; Jiang, Haobo; Prakash, Om. "Solution Structure and Expression Profile of an Insect Cytokine: Manduca sexta Stress Response Peptide-2" Protein Pept. Lett. 24, 3-11 (2017).
PubMed: 27903232

Assembly members:

Assembly members:
Stress_Response_Peptide-2, polymer, 25 residues, 2751.12 Da.

Natural source:

Natural source: Common Name: tobacco hornworm Taxonomy ID: 7130 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Manduca Sexta

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Stress_Response_Peptide-2: FGVKDGKCPSGRVRRLGICV PDDDY

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	148

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Stress Response Peptide-2	1

Entities:

Entity 1, Stress Response Peptide-2 25 residues - 2751.12 Da.

1

PHE

GLY

VAL

LYS

ASP

GLY

LYS

CYS

PRO

SER

2

GLY

ARG

VAL

ARG

LEU

GLY

ILE

CYS

VAL

3

PRO

ASP

TYR

Samples:

sample_1: Stress Response Peptide-2 2.2 mM

sample_conditions_1: pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ v3.2, Varian - collection

CCPNMR_Analysis v2.4, Vranken - chemical shift assignment, peak picking

SPARKY v3, Goddard - chemical shift assignment, peak picking, processing

CNS v1.3, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

NMR spectrometers:

Varian Oxford 500 MHz