BMRB Entry 26850

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26850

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Title:
Im7_6-45
Deposition date:
2016-07-13
Original release date:
2016-11-23
Authors:
Salmon, Loic; Ahlstrom, Logan; Horowitz, Scott; Dickson, Alex; Brooks, Charles; Bardwell, James
Citation:

Citation: Salmon, Loic; Ahlstrom, Logan; Horowitz, Scott; Dickson, Alex; Brooks, Charles; Bardwell, James. "Capturing a Dynamic Chaperone-Substrate Interaction Using NMR-Informed Molecular Modeling"  J. Am. Chem. Soc. 138, 9826-9839 (2016).
PubMed: 27415450

Assembly members:

Assembly members:
Im7_6-45, polymer, 40 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: im7_6-45

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Im7_6-45: SISDYTEAEFVQLLKEIEKE NVAATDDVLDVLLEHFVKIT

Data sets:
Data typeCount
13C chemical shifts219
15N chemical shifts73
1H chemical shifts73

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Im7_6-451

Entities:

Entity 1, Im7_6-45 40 residues - Formula weight is not available

1   SERILESERASPTYRTHRGLUALAGLUPHE
2   VALGLNLEULEULYSGLUILEGLULYSGLU
3   ASNVALALAALATHRASPASPVALLEUASP
4   VALLEULEUGLUHISPHEVALLYSILETHR

Samples:

sample_1: Im7_6-45, [U-100% 13C; U-100% 15N], 1 mM; H20 90%; D20 10%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K

sample_conditions_2: pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_2
3D HNCOsample_1isotropicsample_conditions_2
3D HCACOsample_1isotropicsample_conditions_2
3D HNCAsample_1isotropicsample_conditions_2
3D HN(CO)CAsample_1isotropicsample_conditions_2
3D CBCA(CO)NHsample_1isotropicsample_conditions_2
3D HNCACBsample_1isotropicsample_conditions_2

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Agilent INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks