BMRB Entry 26843

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for NCP15 nucleocapsid
Published: 2016-08-23

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26843

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for NCP15 nucleocapsid

Deposition date:

2016-07-04

Original release date:

2016-08-23

Authors:

LARUE, Valery; TISNE, Carine

Citation:

Citation: Larue, Valery; Catala, Marjorie; Belfetmi, Anissa; Zargarian, Loussine; Mauffret, Olivier; Tisne, Carine. "." Biomol. NMR Assign. 12, 139-143 (2018).
PubMed: 29332151

Assembly members:

Assembly members:
HIV-1_NCP15_Nucleocapsid, polymer, 123 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: HIV Taxonomy ID: 12721 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus HIV

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HIV-1_NCP15_Nucleocapsid: IQKGNFRNQRKTVKCFNCGK EGHIAKNCRAPRKKGCWKCG KEGHQMKDCTERQANFLGKI WPSHKGRPGNFLQSRPEPTA PPEESFRFGEETTTPSQKQE PIDKELYPLASLRSLFGSDP SSQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	296
15N chemical shifts	104
1H chemical shifts	283

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NCP15	1
2	ZnCl2	2

Entities:

Entity 1, NCP15 123 residues - Formula weight is not available

1

ILE

GLN

LYS

GLY

ASN

PHE

ARG

ASN

GLN

ARG

2

LYS

THR

VAL

LYS

CYS

PHE

ASN

CYS

GLY

LYS

3

GLU

GLY

HIS

ILE

ALA

LYS

ASN

CYS

ARG

ALA

4

PRO

ARG

LYS

GLY

CYS

TRP

LYS

CYS

GLY

5

LYS

GLU

GLY

HIS

GLN

MET

LYS

ASP

CYS

THR

6

GLU

ARG

GLN

ALA

ASN

PHE

LEU

GLY

LYS

ILE

7

TRP

PRO

SER

HIS

LYS

GLY

ARG

PRO

GLY

ASN

8

PHE

LEU

GLN

SER

ARG

PRO

GLU

PRO

THR

ALA

9

PRO

GLU

SER

PHE

ARG

PHE

GLY

GLU

10

GLU

THR

PRO

SER

GLN

LYS

GLN

GLU

11

PRO

ILE

ASP

LYS

GLU

LEU

TYR

PRO

LEU

ALA

12

SER

LEU

ARG

SER

LEU

PHE

GLY

SER

ASP

PRO

13

SER

GLN

Entity 2, ZnCl2 - 65.409 Da.

1

ZN

Samples:

sample_1: NCP15 Nucleocapsid, [U-100% 13C; U-100% 15N], 0.8 mM; acetate buffer 25 mM; NaCl 25 mM; ZnCl2 0.1 mM; Mercaptoethanol 0.1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 25 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 950 MHz
Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks