BMRB Entry 26839

Name: Resonance assignments of the charge assembly helix from the murine leukemia virus
Published: 2016-08-08

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26839

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Resonance assignments of the charge assembly helix from the murine leukemia virus

Deposition date:

2016-06-25

Original release date:

2016-08-08

Authors:

Dolezal, Michal

Citation:

Citation: Dolezal, Michal; Hahravova, Romana; Kozisek, Milan; Bednarova, Lucie; Langerova, Hana; Ruml, Tomas; Rumlova, Michaela. "Functional and Structural Characterization of Novel Type of Linker Connecting Capsid and Nucleocapsid Protein Domains in Murine Leukemia Virus" J. Biol. Chem. 291, 20630-20642 (2016).
PubMed: 27514744

Assembly members:

Assembly members:
MLV-CAH, polymer, 72 residues, Formula weight is not available

Natural source:

Natural source: Common Name: murine leukemia virus Taxonomy ID: 11786 Superkingdom: Viruses Kingdom: not available Genus/species: gammaretrovirus not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MLV-CAH: SYYHHHHHHDYDIPTTENLY FQGAMAKRETPEEREERIRR EIEEKEERRRAEDEQRERER DRRRHREMSKLL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	203
15N chemical shifts	69
1H chemical shifts	236

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MLV-CAH	1

Entities:

Entity 1, MLV-CAH 72 residues - Formula weight is not available

residues 1-26 are an affinity tag

1

SER

TYR

HIS

ASP

2

TYR

ASP

ILE

PRO

THR

GLU

ASN

LEU

TYR

3

PHE

GLN

GLY

ALA

MET

ALA

LYS

ARG

GLU

THR

4

PRO

GLU

ARG

GLU

ARG

ILE

ARG

5

GLU

ILE

GLU

LYS

GLU

ARG

6

ALA

GLU

ASP

GLU

GLN

ARG

GLU

ARG

GLU

ARG

7

ASP

ARG

HIS

ARG

GLU

MET

SER

LYS

8

LEU

Samples:

sample_1: MLV-CAH, [U-99% 13C; U-99% 15N], 0.6 mM; sodium phosphate 50 mM; sodium chloride 50 mM; TCEP 1 mM; H2O 95%; D2O, [U-2H], 5%

sample_conditions_1: ionic strength: 0.350 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D CACO	sample_1	isotropic	sample_conditions_1
2D CON	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNN	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D CONCA	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr_Analysis v2.3, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks