BMRB Entry 26820

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR26820

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Title:
Lunasin assignment (truncated form 16-43)
Deposition date:
2016-06-20
Original release date:
2016-07-20
Authors:
Aleksis, Rihards; Jaudzems, Kristaps; Muceniece, Ruta; Liepinsh, Edvards
Citation:

Citation: Aleksis, Rihards; Jaudzems, Kristaps; Muceniece, Ruta; Liepinsh, Edvards. "Lunasin is a redox sensitive intrinsically disordered peptide with two transiently populated \u03b1-helical regions"  Peptides 85, 56-62 (2016).
PubMed: 27639324

Assembly members:

Assembly members:
Lunasin16-43, polymer, 28 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETDuet-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Lunasin16-43: GVNLTPCEKHIMEKIQGRGD DDDDDDDD

Data sets:
Data typeCount
13C chemical shifts157
15N chemical shifts58
1H chemical shifts344

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Lunasin16-431

Entities:

Entity 1, Lunasin16-43 28 residues - Formula weight is not available

1   GLYVALASNLEUTHRPROCYSGLULYSHIS
2   ILEMETGLULYSILEGLNGLYARGGLYASP
3   ASPASPASPASPASPASPASPASP

Samples:

sample_1: Lunasin16-43, [U-99% 15N], 1 mM; D2O, [U-99% 2H], 7%; H2O 93%; sodium phosphate 20 mM; sodium azide 0.03%

pH35: ionic strength: 20 mM; pH: 3.5; pressure: 1 atm; temperature: 273 K

pH65: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicpH35
2D 1H-1H TOCSYsample_1isotropicpH35
2D 1H-1H NOESYsample_1isotropicpH35
3D 1H-15N NOESYsample_1isotropicpH35
2D 1H-13C HSQC aliphaticsample_1isotropicpH35
2D 1H-15N HSQCsample_1isotropicpH65
2D 1H-1H TOCSYsample_1isotropicpH65
2D 1H-1H NOESYsample_1isotropicpH65
3D 1H-15N NOESYsample_1isotropicpH65
2D 1H-13C HSQC aliphaticsample_1isotropicpH65

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks