BMRB Entry 26806

Name: Chemical Shift Assignment of Tom1 VHS Domain
Published: 2016-10-13

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26806

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical Shift Assignment of Tom1 VHS Domain

Deposition date:

2016-05-23

Original release date:

2016-10-13

Authors:

Capelluto, Daniel; Shanaiah, Narasimhamurthy; Ellena, Jeffrey

Citation:

Citation: Ellena, Jeffrey; Xiong, Wen; Zhao, Xiaolin; Shanaiah, Narasimhamurthy; Capelluto, Daniel. "Backbone 1H, 15N, and 13C resonance assignments of the Tom1 VHS domain" Biomol. NMR Assign. 11, 1-4 (2017).
PubMed: 27704363

Assembly members:

Assembly members:
Tom1-VHS, polymer, 139 residues, 15563.88 Da.

Natural source:

Natural source: Common Name: humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX4T3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Tom1-VHS: SPVGQRIEKATDGSLQSEDW ALNMEICDIINETEEGPKDA LRAVKKRIVGNKNFHEVMLA LTVLETCVKNCGHRFHVLVA SQDFVESVLVRTILPKNNPP TIVHDKVLNLIQSWADAFRS SPDLTGVVTIYEDLRRKGL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	380
15N chemical shifts	124
1H chemical shifts	124

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Tom1-VHS	1

Entities:

Entity 1, Tom1-VHS 139 residues - 15563.88 Da.

1

SER

PRO

VAL

GLY

GLN

ARG

ILE

GLU

LYS

ALA

2

THR

ASP

GLY

SER

LEU

GLN

SER

GLU

ASP

TRP

3

ALA

LEU

ASN

MET

GLU

ILE

CYS

ASP

ILE

4

ASN

GLU

THR

GLU

GLY

PRO

LYS

ASP

ALA

5

LEU

ARG

ALA

VAL

LYS

ARG

ILE

VAL

GLY

6

ASN

LYS

ASN

PHE

HIS

GLU

VAL

MET

LEU

ALA

7

LEU

THR

VAL

LEU

GLU

THR

CYS

VAL

LYS

ASN

8

CYS

GLY

HIS

ARG

PHE

HIS

VAL

LEU

VAL

ALA

9

SER

GLN

ASP

PHE

VAL

GLU

SER

VAL

LEU

VAL

10

ARG

THR

ILE

LEU

PRO

LYS

ASN

PRO

11

THR

ILE

VAL

HIS

ASP

LYS

VAL

LEU

ASN

LEU

12

ILE

GLN

SER

TRP

ALA

ASP

ALA

PHE

ARG

SER

13

SER

PRO

ASP

LEU

THR

GLY

VAL

THR

ILE

14

TYR

GLU

ASP

LEU

ARG

LYS

GLY

LEU

Samples:

Tom1-VHS_sam: Tom1-VHS, [U-13C; U-15N], 800 uM; H2O 90%; D2O 10%

Tom1-VHS_con: pH: 7; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	Tom1-VHS_sam	isotropic	Tom1-VHS_con
3D HNCACB	Tom1-VHS_sam	isotropic	Tom1-VHS_con
3D CBCA(CO)NH	Tom1-VHS_sam	isotropic	Tom1-VHS_con
3D HNCA	Tom1-VHS_sam	isotropic	Tom1-VHS_con
3D HNCO	Tom1-VHS_sam	isotropic	Tom1-VHS_con

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking

Sparky, Goddard - processing

NMRview, Johnson, One Moon Scientific - processing

CCPNMR, Wayne Boucher and Tim Stevens - chemical shift assignment

NMR spectrometers:

Bruker Avance III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks