BMRB Entry 26791

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26791

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Title:
Backbone 1H, 13C, and 15 N Chemical Shift Assignment for human Langerin CRD
Deposition date:
2016-05-09
Original release date:
2016-08-30
Authors:
Hanske, Jonas; Rademacher, Christoph; Schmieder, Peter; Jurk, Marcel; Ballaschk, Martin; Beerbaum, Monika; Aleksic, Stevan; Keller, Bettina; Shanina, Elena
Citation:

Citation: Hanske, Jonas; Aleksic, Stevan; Ballaschk, Martin; Jurk, Marcel; Shanina, Elena; Beerbaum, Monika; Schmieder, Peter; Keller, Bettina; Rademacher, Christoph. "An intra-domain allosteric network modulates the Ca2+ affinity in C-type lectin receptor Langerin"  J. Am. Chem. Soc. 138, 12176-12186 (2016).
PubMed: 27560542

Assembly members:

Assembly members:
LangCRD, polymer, 157 residues, 17960 Da.
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET30a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LangCRD: MAQVVSQGWKYFKGNFYYFS LIPKTWYSAEQFCVSRNSHL TSVTSESEQEFLYKTAGGLI YWIGLTKAGMEGDWSWVDDT PFNKVQSARFWIPGEPNNAG NNEHCGNIKAPSLQAWNDAP CDKTFLFICKRPYVPSEPGS ENLYFQGSAWSHPQFEK

Data typeCount
13C chemical shifts463
15N chemical shifts150
1H chemical shifts247
T1 relaxation values354
T2 relaxation values354
coupling constants152
heteronuclear NOE values353

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1CRD1
2CALCIUM ION2

Entities:

Entity 1, CRD 157 residues - 17960 Da.

Residues 329-347 represent a non-native affinity tag This is the extracellular carbohydrate recognition domain of a membrane receptor

1   METALAGLNVALVALSERGLNGLYTRPLYS
2   TYRPHELYSGLYASNPHETYRTYRPHESER
3   LEUILEPROLYSTHRTRPTYRSERALAGLU
4   GLNPHECYSVALSERARGASNSERHISLEU
5   THRSERVALTHRSERGLUSERGLUGLNGLU
6   PHELEUTYRLYSTHRALAGLYGLYLEUILE
7   TYRTRPILEGLYLEUTHRLYSALAGLYMET
8   GLUGLYASPTRPSERTRPVALASPASPTHR
9   PROPHEASNLYSVALGLNSERALAARGPHE
10   TRPILEPROGLYGLUPROASNASNALAGLY
11   ASNASNGLUHISCYSGLYASNILELYSALA
12   PROSERLEUGLNALATRPASNASPALAPRO
13   CYSASPLYSTHRPHELEUPHEILECYSLYS
14   ARGPROTYRVALPROSERGLUPROGLYSER
15   GLUASNLEUTYRPHEGLNGLYSERALATRP
16   SERHISPROGLNPHEGLULYS

Entity 2, CALCIUM ION - 40.078 Da.

1   CA

Related Database Links:

PDB 3p5f

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SPARKY: Backbone or all simulated peaks