BMRB Entry 26777

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26777

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Title:
1H, 13C and 15N resonance assignments for the response regulator CheY3 from Rhodobacter spheroids at pH 4.5 with BeF3-
Deposition date:
2016-04-12
Original release date:
2016-09-02
Authors:
Varela-Alvarez, Lorena; Bell, Christian; Armitage, Judith; Redfield, Christina
Citation:

Citation: Varela-Alvarez, Lorena; Bell, Christian; Armitage, Judith; Redfield, Christina. "1H, 13C and 15N resonance assignments for the response regulator CheY3 from Rhodobacter sphaeroides"  Biomol. NMR Assign. 10, 373-378 (2016).
PubMed: 27468962

Assembly members:

Assembly members:
CheY3_BeF3_pH4.5, polymer, 132 residues, Formula weight is not available
entity_MG, non-polymer, 24.305 Da.
entity_BEF, non-polymer, 66.007 Da.

Natural source:

Natural source:   Common Name: Rhodobacter sphaeroides   Taxonomy ID: 1063   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Rhodobacter sphaeroides

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE-80

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CheY3_BeF3_pH4.5: MRGSHHHHHHGSSRTVLAVD DSPSVRSMVAMTLREAGYRV IEATDGREGLEKALSEPVDA IITDQNMPNLDGLGFIRAFR EHPESKGKPIIFLSTDSADT LKQQAREAGAMGWMVKPFTQ PQLLAVIKKVLG

Data sets:
Data typeCount
13C chemical shifts538
15N chemical shifts125
1H chemical shifts838

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CheY31
2Mg2+2
3BeF3-3

Entities:

Entity 1, CheY3 132 residues - Formula weight is not available

The protein contains an N-terminal His6 tag preceded by MRGS. We are providing only some assignments for the non-native tag residues.

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERSERARGTHRVALLEUALAVALASP
3   ASPSERPROSERVALARGSERMETVALALA
4   METTHRLEUARGGLUALAGLYTYRARGVAL
5   ILEGLUALATHRASPGLYARGGLUGLYLEU
6   GLULYSALALEUSERGLUPROVALASPALA
7   ILEILETHRASPGLNASNMETPROASNLEU
8   ASPGLYLEUGLYPHEILEARGALAPHEARG
9   GLUHISPROGLUSERLYSGLYLYSPROILE
10   ILEPHELEUSERTHRASPSERALAASPTHR
11   LEULYSGLNGLNALAARGGLUALAGLYALA
12   METGLYTRPMETVALLYSPROPHETHRGLN
13   PROGLNLEULEUALAVALILELYSLYSVAL
14   LEUGLY

Entity 2, Mg2+ - Mg - 24.305 Da.

1   MG

Entity 3, BeF3- - Be F3 - 66.007 Da.

1   BEF

Samples:

sample_1_15N: CheY3_BeF3_pH4.5, [U-99% 15N], 2 mM; Mg2+ 2 mM; BeF3- 4.5 mM; Sodium Acetate 20 mM

sample_2_15N_13C: CheY3_BeF3_pH4.5, [U-99% 13C; U-99% 15N], 2 mM; Mg2+ 2 mM; BeF3- 4.5 mM; Sodium Acetate 20 mM

sample_conditions_1: pH: 4.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1_15Nisotropicsample_conditions_1
3D 1H-15N NOESYsample_1_15Nisotropicsample_conditions_1
2D 1H-15N HSQCsample_2_15N_13Cisotropicsample_conditions_1
3D HNCAsample_2_15N_13Cisotropicsample_conditions_1
3D CBCANHsample_2_15N_13Cisotropicsample_conditions_1
3D CBCA(CO)NHsample_2_15N_13Cisotropicsample_conditions_1
3D HBHA(CO)NHsample_2_15N_13Cisotropicsample_conditions_1
3D HCCH-TOCSYsample_2_15N_13Cisotropicsample_conditions_1
3D HNCACOsample_2_15N_13Cisotropicsample_conditions_1
3D HNCOsample_2_15N_13Cisotropicsample_conditions_1
3D 1H-15N TOCSYsample_1_15Nisotropicsample_conditions_1
3D (H)C(CCO)NHsample_2_15N_13Cisotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2_15N_13Cisotropicsample_conditions_1
3D H(CCO)NHsample_2_15N_13Cisotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNmr_Analysis, CCPN - data analysis

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Home-built OMEGA 750 MHz
  • Home-built OMEGA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks