BMRB Entry 26763

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26763

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Title:
1H, 13C, and 15N backbone chemical shift assignments of StAR-related lipid transfer domain protein 6 (STARD6) bound to testosterone
Deposition date:
2016-03-21
Original release date:
2016-04-22
Authors:
Letourneau, Danny; Bedard, Mikael; Cabana, Jerome; Lefebvre, Andree; Lehoux, Jean-Guy; Lavigne, Pierre
Citation:

Citation: Letourneau, Danny; Bedard, Mikael; Cabana, Jerome; Lefebvre, Andree; Lehoux, Jean-Guy; Lavigne, Pierre. "STARD6 on steroids: solution structure, multiple timescale backbone dynamics and ligand binding mechanism"  Sci. Rep. 6, 28486-28486 (2016).
PubMed: 27340016

Assembly members:

Assembly members:
STARD6, polymer, 226 residues, Formula weight is not available
TESTOSTERONE, non-polymer, 288.424 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
STARD6: MDFKAIAQQTAQEVLGYNRD TSGWKVVKTSKKITVSSKAS RKFHGNLYRVEGIIPESPAK LSDFLYQTGDRITWDKSLQV YNMVHRIDSDTFICHTITQS FAVGSISPRDFIDLVYIKRY EGNMNIISSKSVDFPEYPPS SNYIRGYNHPCGFVCSPMEE NPAYSKLVMFVQTEMRGKLS PSIIEKTMPSNLVNFILNAK DGIKAHRTPSRRGFHHNSHS HHHHHH

Data sets:
Data typeCount
13C chemical shifts608
15N chemical shifts201
1H chemical shifts201

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1STARD61
2testosterone2

Entities:

Entity 1, STARD6 226 residues - Formula weight is not available

1   METASPPHELYSALAILEALAGLNGLNTHR
2   ALAGLNGLUVALLEUGLYTYRASNARGASP
3   THRSERGLYTRPLYSVALVALLYSTHRSER
4   LYSLYSILETHRVALSERSERLYSALASER
5   ARGLYSPHEHISGLYASNLEUTYRARGVAL
6   GLUGLYILEILEPROGLUSERPROALALYS
7   LEUSERASPPHELEUTYRGLNTHRGLYASP
8   ARGILETHRTRPASPLYSSERLEUGLNVAL
9   TYRASNMETVALHISARGILEASPSERASP
10   THRPHEILECYSHISTHRILETHRGLNSER
11   PHEALAVALGLYSERILESERPROARGASP
12   PHEILEASPLEUVALTYRILELYSARGTYR
13   GLUGLYASNMETASNILEILESERSERLYS
14   SERVALASPPHEPROGLUTYRPROPROSER
15   SERASNTYRILEARGGLYTYRASNHISPRO
16   CYSGLYPHEVALCYSSERPROMETGLUGLU
17   ASNPROALATYRSERLYSLEUVALMETPHE
18   VALGLNTHRGLUMETARGGLYLYSLEUSER
19   PROSERILEILEGLULYSTHRMETPROSER
20   ASNLEUVALASNPHEILELEUASNALALYS
21   ASPGLYILELYSALAHISARGTHRPROSER
22   ARGARGGLYPHEHISHISASNSERHISSER
23   HISHISHISHISHISHIS

Entity 2, testosterone - 288.424 Da.

1   TES

Samples:

sample_1: STARD6, [U-100% 15N], 0.8 – 1.1 mM; Testosterone 1.2 mM; potassium phosphate 50 mM; potassium chloride 50 mM; TCEP 1 mM; sodium azide 0.02%; D2O 10%; ethanol 2%

sample_conditions_1: ionic strength: 100 mM; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR-STAR, CCPN - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks