BMRB Entry 26762

Name: Elk1 C-terminus aa309-429
Published: 2017-08-24

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26762

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Elk1 C-terminus aa309-429

Deposition date:

2016-03-20

Original release date:

2017-08-24

Authors:

Theillet, Francois; Selenko, Philipp; Mylona, Anastasia; Treisman, Richard

Citation:

Citation: Mylona, Anastasia; Theillet, Francois-Xavier; Foster, Charles; Cheng, Tammy; Miralles, Francesc; Bates, Paul; Selenko, Philipp; Treisman, Richard. "Opposing effects of Elk-1 multisite phosphorylation shape its response to ERK activation" Science 354, 233-237 (2016).
PubMed: 27738173

Assembly members:

Assembly members:
Elk1, polymer, 122 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-29

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Elk1: GTQPQKGRKPRDLELPLSPS LLGGQGPERTPGSGTSSGLQ APGPALTPSLLPTHTLTPVL LTPSSLPPSIHFWSTLSPIA PRSPAKLSFQFPSSGSAQVH IPSISVDGLSTPVVLSPGPQ KP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	321
15N chemical shifts	97
1H chemical shifts	97

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Elk1	1

Entities:

Entity 1, Elk1 122 residues - Formula weight is not available

There is one supplementary glycine at the N-terminus (for improved TEV cleavage of the initial construct).

1

GLY

THR

GLN

PRO

GLN

LYS

GLY

ARG

LYS

PRO

2

ARG

ASP

LEU

GLU

LEU

PRO

LEU

SER

PRO

SER

3

LEU

GLY

GLN

GLY

PRO

GLU

ARG

THR

4

PRO

GLY

SER

GLY

THR

SER

GLY

LEU

GLN

5

ALA

PRO

GLY

PRO

ALA

LEU

THR

PRO

SER

LEU

6

LEU

PRO

THR

HIS

THR

LEU

THR

PRO

VAL

LEU

7

LEU

THR

PRO

SER

LEU

PRO

SER

ILE

8

HIS

PHE

TRP

SER

THR

LEU

SER

PRO

ILE

ALA

9

PRO

ARG

SER

PRO

ALA

LYS

LEU

SER

PHE

GLN

10

PHE

PRO

SER

GLY

SER

ALA

GLN

VAL

HIS

11

ILE

PRO

SER

ILE

SER

VAL

ASP

GLY

LEU

SER

12

THR

PRO

VAL

LEU

SER

PRO

GLY

PRO

GLN

13

LYS

PRO

Samples:

sample_1: Elk1, [U-99% 13C; U-99% 15N], 400 uM; Na phosphate 10 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.9; pressure: 1 atm; temperature: 277 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.1, Bruker Biospin - collection, processing

CcpNMR_Analysis v2.4, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks