BMRB Entry 26755

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26755

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for the intrinsically disordered NHE1 distal tail
Deposition date:
2016-03-11
Original release date:
2016-03-16
Authors:
Hendus-Altenburger, Ruth; Kragelund, Birthe; Pedersen, Stine; Norholm, Ann-Beth
Citation:

Citation: Hendus-Altenburger, Ruth; Pedraz-Cuesta, Elena; Olesen, Christina; Papaleo, Elena; Schnell, Jeff; Hopper, Jonathan; Robinson, Carol; Pedersen, Stine; Kragelund, Birthe. "The human Na(+)/H(+) exchanger 1 is a membrane scaffold protein for extracellular signal-regulated kinase 2"  BMC Biol. 14, .-. (2016).
PubMed: 27083547

Assembly members:

Assembly members:
NHE1, polymer, 137 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NHE1: MINNYLTVPAHKLDSPTMSR ARIGSDPLAYEPKEDLPVIT IDPASPQSPESVDLVNEELK GKVLGLSRDPAKVAEEDEDD DGGIMMRSKETSSPGTDDVF TPAPSDSPSSQRIQRCLSDP GPHPEPGEGEPFFPKGQ

Data sets:
Data typeCount
13C chemical shifts381
15N chemical shifts113
1H chemical shifts113

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NHE1, chain 11
2NHE1, chain 21

Entities:

Entity 1, NHE1, chain 1 137 residues - Formula weight is not available

Artificial Met at the beginning, I680-Q815 are from human NHE1 (SLC9A1)

1   METILEASNASNTYRLEUTHRVALPROALA
2   HISLYSLEUASPSERPROTHRMETSERARG
3   ALAARGILEGLYSERASPPROLEUALATYR
4   GLUPROLYSGLUASPLEUPROVALILETHR
5   ILEASPPROALASERPROGLNSERPROGLU
6   SERVALASPLEUVALASNGLUGLULEULYS
7   GLYLYSVALLEUGLYLEUSERARGASPPRO
8   ALALYSVALALAGLUGLUASPGLUASPASP
9   ASPGLYGLYILEMETMETARGSERLYSGLU
10   THRSERSERPROGLYTHRASPASPVALPHE
11   THRPROALAPROSERASPSERPROSERSER
12   GLNARGILEGLNARGCYSLEUSERASPPRO
13   GLYPROHISPROGLUPROGLYGLUGLYGLU
14   PROPHEPHEPROLYSGLYGLN

Related Database Links:

NCBI P19634

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks