BMRB Entry 26748

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26748

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Title:
NMR study of Met-1 Human Angiogenin - 1H, 13C, 15N backbone and side chain resonance assignment
Deposition date:
2016-02-19
Original release date:
2016-09-29
Authors:
Tsika, Aikaterini; Chatzileontiadou, Dimitra; Leonidas, Demetres; Spyroulias, Georgios
Citation:

Citation: Tsika, Aikaterini; Chatzileontiadou, Dimitra; Leonidas, Dimitra; Spyroulias, Georgios. "NMR study of Met-1 human Angiogenin: (1)H, (13)C, (15)N backbone and side-chain resonance assignment"  Biomol. NMR Assign. 10, 379-383 (2016).
PubMed: 27624767

Assembly members:

Assembly members:
Met-1_Human_Ang, polymer, 124 residues, 14274.1 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Met-1_Human_Ang: MQDNSRYTHFLTQHYDAKPQ GRDDRYCESIMRRRGLTSPC KDINTFIHGNKRSIKAICEN KNGNPHRENLRISKSSFQVT TCKLHGGSPWPPCQYRATAG FRNVVVACENGLPVHLDQSI FRRP

Data sets:
Data typeCount
13C chemical shifts469
15N chemical shifts112
1H chemical shifts714

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Met-1 Human Ang1

Entities:

Entity 1, Met-1 Human Ang 124 residues - 14274.1 Da.

1   METGLNASPASNSERARGTYRTHRHISPHE
2   LEUTHRGLNHISTYRASPALALYSPROGLN
3   GLYARGASPASPARGTYRCYSGLUSERILE
4   METARGARGARGGLYLEUTHRSERPROCYS
5   LYSASPILEASNTHRPHEILEHISGLYASN
6   LYSARGSERILELYSALAILECYSGLUASN
7   LYSASNGLYASNPROHISARGGLUASNLEU
8   ARGILESERLYSSERSERPHEGLNVALTHR
9   THRCYSLYSLEUHISGLYGLYSERPROTRP
10   PROPROCYSGLNTYRARGALATHRALAGLY
11   PHEARGASNVALVALVALALACYSGLUASN
12   GLYLEUPROVALHISLEUASPGLNSERILE
13   PHEARGARGPRO

Samples:

sample_1: Met-1 Human Ang, [U-99% 15N], 0.6 mM; Sodium Acetate 50 mM; EDTA 0.2 mM

sample_2: Met-1 Human Ang, [U-99% 13C; U-99% 15N], 0.6 mM; Sodium Acetate 50 mM; EDTA 0.2 mM

sample_3: Met-1 Human Ang, [U-99% 15N], 0.3 mM; Sodium Acetate 50 mM; EDTA 0.2 mM

sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 298 K

sample_conditions_2: pH: 5; pressure: 1 atm; temperature: 298 K

sample_conditions_3: pH: 5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_3
2D 1H-13C HSQCsample_2isotropicsample_conditions_2
3D CBCA(CO)NHsample_2isotropicsample_conditions_2
3D HNCACBsample_2isotropicsample_conditions_2
3D HNCAsample_2isotropicsample_conditions_2
3D HNCOsample_2isotropicsample_conditions_2
3D HN(CO)CAsample_2isotropicsample_conditions_2
3D HCCH-TOCSYsample_2isotropicsample_conditions_2
3D HBHA(CO)NHsample_2isotropicsample_conditions_2
2D 1H-13C HSQCsample_2isotropicsample_conditions_2
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_2
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_2
3D HNHAsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_2
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_2
3D H(CA)COsample_2isotropicsample_conditions_2
2D 1H-15N HSQCsample_2isotropicsample_conditions_2

Software:

TOPSPIN v3.2 PI6, Bruker Biospin - collection, processing

CARA v1.5.5, ROCHUS KELLER - chemical shift assignment

NMR spectrometers:

  • Bruker Avance HD-III HD 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks