BMRB Entry 26735

Name: LdcI Assocating Domain of RavA Backbone Assignment
Published: 2016-06-08

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26735

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

LdcI Assocating Domain of RavA Backbone Assignment

Deposition date:

2016-01-23

Original release date:

2016-06-08

Authors:

Sharpe, Simon; Houry, Walid A.; Chan, Sze Wah Samuel

Citation:

Citation: Chan, Sze Wah Samuel. "Mechanism of Amyloidogenesis of a Bacterial AAA+ Chaperone" Structure 24, 1095-1109 (2016).
PubMed: 27265850

Assembly members:

Assembly members:
LARA3, polymer, 115 residues, 12609.5014 Da.

Natural source:

Natural source: Common Name: Escherichia coli (strain K12) Taxonomy ID: 511693 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: p11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LARA3: GHMQQSDKTALTVIRLGGIF SRRQQYQLPVNVTASTLTLL LQKPLKLHDMEVVHISFERS ALEQWLSKGGEIRGKLNGIG FAQKLNLEVDSAQHLVVRDV SLQGSTLALPGSSAE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	359
15N chemical shifts	111
1H chemical shifts	111

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	LARA3	1

Entities:

Entity 1, LARA3 115 residues - 12609.5014 Da.

1

GLY

HIS

MET

GLN

SER

ASP

LYS

THR

ALA

2

LEU

THR

VAL

ILE

ARG

LEU

GLY

ILE

PHE

3

SER

ARG

GLN

TYR

GLN

LEU

PRO

VAL

4

ASN

VAL

THR

ALA

SER

THR

LEU

THR

LEU

5

LEU

GLN

LYS

PRO

LEU

LYS

LEU

HIS

ASP

MET

6

GLU

VAL

HIS

ILE

SER

PHE

GLU

ARG

SER

7

ALA

LEU

GLU

GLN

TRP

LEU

SER

LYS

GLY

8

GLU

ILE

ARG

GLY

LYS

LEU

ASN

GLY

ILE

GLY

9

PHE

ALA

GLN

LYS

LEU

ASN

LEU

GLU

VAL

ASP

10

SER

ALA

GLN

HIS

LEU

VAL

ARG

ASP

VAL

11

SER

LEU

GLN

GLY

SER

THR

LEU

ALA

LEU

PRO

12

GLY

SER

ALA

GLU

Samples:

LARA: LARA3, [[U-95% 13C; U-95% 15N]], 0.45 mM; H2O 90%; D2O 10%; potassium chloride 50 mM

Assignment: ionic strength: 50.000 mM; pH: 4.000; pressure: 1.000 atm; temperature: 280.150 K

Experiments:

Name	Sample	Sample state	Sample conditions
COCBCA (H[N[co[{CA\|ca[C]}]]])	LARA	isotropic	Assignment
CCCONH (C_ccoNH.relayed)	LARA	isotropic	Assignment
3D HNCACB	LARA	isotropic	Assignment
CACO (H[N[ca[CO]]])	LARA	isotropic	Assignment
3D HNCA	LARA	isotropic	Assignment
3D HN(CO)CA	LARA	isotropic	Assignment
3D HNCO	LARA	isotropic	Assignment
2D 1H-15N HSQC/HMQC	LARA	isotropic	Assignment

Software:

CcpNmr_Analysis v2.3, CCPN - Aid

NMR spectrometers:

Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks