BMRB Entry 26731
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26731
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Perez-Borrajero, Cecilia; Okon, Mark; McIntosh, Lawrence. "Structural and Dynamics Studies of Pax5 Reveal Asymmetry in Stability and DNA Binding by the Paired Domain" J. Mol. Biol. 428, 2372-2391 (2016).
PubMed: 27067111
Assembly members:
Paired_domain_of_Pax5, polymer, 151 residues, Formula weight is not available
DNA_1, polymer, 25 residues, Formula weight is not available
DNA_2, polymer, 25 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28-MHL
Entity Sequences (FASTA):
Paired_domain_of_Pax5: GHMDLEKNYPTPRTSRTGHG
GVNQLGGVFVNGRPLPDVVR
QRIVELAHQGVRPCDISRQL
RVSHGCVSKILGRYYETGSI
KPGVIGGSKPKVATPKVVEK
IAEYKRQNPTMFAWEIRDRL
LAERVCDNDTVPSVSSINRI
IRTKVQQPPNQ
DNA_1: CGGTGGTCACGCCTCAGTGC
CCCAT
DNA_2: GCCACCAGTGCGGAGTCACG
GGGTA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 424 |
| 15N chemical shifts | 135 |
| 1H chemical shifts | 135 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Paired domain of Pax5 | 1 |
| 2 | DNA_1 | 2 |
| 3 | DNA_2 | 3 |
Entities:
Entity 1, Paired domain of Pax5 151 residues - Formula weight is not available
| 1 | GLY | HIS | MET | ASP | LEU | GLU | LYS | ASN | TYR | PRO | ||||
| 2 | THR | PRO | ARG | THR | SER | ARG | THR | GLY | HIS | GLY | ||||
| 3 | GLY | VAL | ASN | GLN | LEU | GLY | GLY | VAL | PHE | VAL | ||||
| 4 | ASN | GLY | ARG | PRO | LEU | PRO | ASP | VAL | VAL | ARG | ||||
| 5 | GLN | ARG | ILE | VAL | GLU | LEU | ALA | HIS | GLN | GLY | ||||
| 6 | VAL | ARG | PRO | CYS | ASP | ILE | SER | ARG | GLN | LEU | ||||
| 7 | ARG | VAL | SER | HIS | GLY | CYS | VAL | SER | LYS | ILE | ||||
| 8 | LEU | GLY | ARG | TYR | TYR | GLU | THR | GLY | SER | ILE | ||||
| 9 | LYS | PRO | GLY | VAL | ILE | GLY | GLY | SER | LYS | PRO | ||||
| 10 | LYS | VAL | ALA | THR | PRO | LYS | VAL | VAL | GLU | LYS | ||||
| 11 | ILE | ALA | GLU | TYR | LYS | ARG | GLN | ASN | PRO | THR | ||||
| 12 | MET | PHE | ALA | TRP | GLU | ILE | ARG | ASP | ARG | LEU | ||||
| 13 | LEU | ALA | GLU | ARG | VAL | CYS | ASP | ASN | ASP | THR | ||||
| 14 | VAL | PRO | SER | VAL | SER | SER | ILE | ASN | ARG | ILE | ||||
| 15 | ILE | ARG | THR | LYS | VAL | GLN | GLN | PRO | PRO | ASN | ||||
| 16 | GLN |
Entity 2, DNA_1 25 residues - Formula weight is not available
| 1 | DC | DG | DG | DT | DG | DG | DT | DC | DA | DC | ||||
| 2 | DG | DC | DC | DT | DC | DA | DG | DT | DG | DC | ||||
| 3 | DC | DC | DC | DA | DT |
Entity 3, DNA_2 25 residues - Formula weight is not available
| 1 | DG | DC | DC | DA | DC | DC | DA | DG | DT | DG | ||||
| 2 | DC | DG | DG | DA | DG | DT | DC | DA | DC | DG | ||||
| 3 | DG | DG | DG | DT | DA |
Samples:
sample_1: Paired domain of Pax5, [U-100% 13C; U-100% 15N; U-80% 2H], 0.35 mM; DNA_1 0.35 mM; DNA_2 0.35 mM; MES 20 mM; NaCl 20 mM; DTT 2 mM; EDTA 0.5 mM
sample_conditions_1: ionic strength: 0.02 M; pH: 6.5; pressure: 1 atm; temperature: 298.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker Avance 850 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks