BMRB Entry 26727

Name: 1H, 13C and 15N assignment for the C-terminal domain of human Doublecortin
Published: 2016-07-14

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26727

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H, 13C and 15N assignment for the C-terminal domain of human Doublecortin

Deposition date:

2016-01-10

Original release date:

2016-07-14

Authors:

Di Lello, Paola

Citation:

Citation: Burger, Dominique; Stihle, Martine; Sharma, Ashwani; Di Lello, Paola; Benz, Jorg; D'Arcy, Brigitte; Debulpaep, Maja; Fry, David; Huber, Walter; Kremer, Thomas; Laeremans, Toon; Matile, Hugues; Ross, Alfred; Rufer, Arne; Schoch, Guillaume; Steinmetz, Michel; Steyaert, Jan; Rudolph, Markus; Thoma, Ralf; Ruf, Armin. "Crystal Structures of the Human Doublecortin C- and N-terminal Domains in Complex with Specific Antibodies" J. Biol. Chem. 291, 16292-16306 (2016).
PubMed: 27226599

Assembly members:

Assembly members:
Doublecortin_C-terminal_domain, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pER2b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Doublecortin_C-terminal_domain: LVPRGSHMARENKDFVRPKL VTIIRSGVKPRKAVRVLLNK KTAHSFEQVLTDITEAIKLE TGVVKKLYTLDGKQVTCLHD FFGDDDVFIACGPEKFRYAQ DDFSLDENECRVMK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	417
15N chemical shifts	108
1H chemical shifts	109

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Doublecortin C-terminal domain monomer	1

Entities:

Entity 1, Doublecortin C-terminal domain monomer 114 residues - Formula weight is not available

1

LEU

VAL

PRO

ARG

GLY

SER

HIS

MET

ALA

ARG

2

GLU

ASN

LYS

ASP

PHE

VAL

ARG

PRO

LYS

LEU

3

VAL

THR

ILE

ARG

SER

GLY

VAL

LYS

PRO

4

ARG

LYS

ALA

VAL

ARG

VAL

LEU

ASN

LYS

5

LYS

THR

ALA

HIS

SER

PHE

GLU

GLN

VAL

LEU

6

THR

ASP

ILE

THR

GLU

ALA

ILE

LYS

LEU

GLU

7

THR

GLY

VAL

LYS

LEU

TYR

THR

LEU

8

ASP

GLY

LYS

GLN

VAL

THR

CYS

LEU

HIS

ASP

9

PHE

GLY

ASP

VAL

PHE

ILE

ALA

10

CYS

GLY

PRO

GLU

LYS

PHE

ARG

TYR

ALA

GLN

11

ASP

PHE

SER

LEU

ASP

GLU

ASN

GLU

CYS

12

ARG

VAL

MET

LYS

Samples:

sample_1: Doublecortin C-terminal domain, [U-99% 13C; U-99% 15N], 0.5 mM; sodium acetate 20 mM; TCEP 5 mM; D2O 5%; H2O 95%

sample_conditions_1: ionic strength: 20 mM; pH: 4.0; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D (HB)CBCA(CO)NNH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Varian INOVA 600 MHz
Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks