BMRB Entry 26724

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PDB ID: 2ncp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26724
MolProbity Validation Chart

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Title:
NMR chemical shift assignments for the C-terminal domain of Tetrahymena Tcb2 in the presence of calcium
Deposition date:
2015-12-28
Original release date:
2016-07-14
Authors:
Kilpatrick, Adina; Gurrola, Theodore; Fowler, C. Andrew
Citation:

Citation: Kilpatrick, Adina; Gurrola, Theodore; Sterner, Robert; Sleister, Heidi; Honts, Jerry; Fowler, C. Andrew. "Backbone and side-chain chemical shift assignments for the C-terminal domain of Tcb2, a cytoskeletal calcium-binding protein from Tetrahymena thermophila"  Biomol. NMR Assign. 10, 281-285 (2016).
PubMed: 27155947

Assembly members:

Assembly members:
Tcb2-C, polymer, 102 residues, Formula weight is not available
entity_CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Tetrahymena thermophila   Taxonomy ID: 5911   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Tetrahymena thermophila

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJ411: T7 promoter, inducible

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Tcb2-C: SSKPKYNPEVEAKLDVARRL FKRYDKDGSGQLQDDEIAGL LKDTYAEMGMSNFTPTKEDV KIWLQMADTNSDGSVSLEEY EDLIIKSLQKAGIRVEKQSL VF

Data sets:
Data typeCount
13C chemical shifts457
15N chemical shifts106
1H chemical shifts679
T1 relaxation values90
T2 relaxation values90
heteronuclear NOE values93

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Tcb2-C1
2CALCIUM ION_12
3CALCIUM ION_22

Entities:

Entity 1, Tcb2-C 102 residues - Formula weight is not available

1   SERSERLYSPROLYSTYRASNPROGLUVAL
2   GLUALALYSLEUASPVALALAARGARGLEU
3   PHELYSARGTYRASPLYSASPGLYSERGLY
4   GLNLEUGLNASPASPGLUILEALAGLYLEU
5   LEULYSASPTHRTYRALAGLUMETGLYMET
6   SERASNPHETHRPROTHRLYSGLUASPVAL
7   LYSILETRPLEUGLNMETALAASPTHRASN
8   SERASPGLYSERVALSERLEUGLUGLUTYR
9   GLUASPLEUILEILELYSSERLEUGLNLYS
10   ALAGLYILEARGVALGLULYSGLNSERLEU
11   VALPHE

Entity 2, CALCIUM ION_1 - Ca - 40.078 Da.

1   CA

Related Database Links:

NCBI P09226.2 XP_001007754.3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks