BMRB Entry 26724

Title:
NMR chemical shift assignments for the C-terminal domain of Tetrahymena Tcb2 in the presence of calcium
Deposition date:
2015-12-28
Original release date:
2016-07-14
Authors:
Kilpatrick, Adina; Gurrola, Theodore; Fowler, C. Andrew
Citation:

Citation: Kilpatrick, Adina; Gurrola, Theodore; Sterner, Robert; Sleister, Heidi; Honts, Jerry; Fowler, C. Andrew. "Backbone and side-chain chemical shift assignments for the C-terminal domain of Tcb2, a cytoskeletal calcium-binding protein from Tetrahymena thermophila"  Biomol. NMR Assign. 10, 281-285 (2016).
PubMed: 27155947

Assembly members:

Assembly members:
Tcb2-C, polymer, 102 residues, Formula weight is not available
entity_CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Tetrahymena thermophila   Taxonomy ID: 5911   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Tetrahymena thermophila

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJ411: T7 promoter, inducible

Data sets:
Data typeCount
13C chemical shifts457
15N chemical shifts106
1H chemical shifts679
T1 relaxation values90
T2 relaxation values90
heteronuclear NOE values93

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tcb2-C1
2CALCIUM ION_12
3CALCIUM ION_22

Entities:

Entity 1, Tcb2-C 102 residues - Formula weight is not available

1   SERSERLYSPROLYSTYRASNPROGLUVAL
2   GLUALALYSLEUASPVALALAARGARGLEU
3   PHELYSARGTYRASPLYSASPGLYSERGLY
4   GLNLEUGLNASPASPGLUILEALAGLYLEU
5   LEULYSASPTHRTYRALAGLUMETGLYMET
6   SERASNPHETHRPROTHRLYSGLUASPVAL
7   LYSILETRPLEUGLNMETALAASPTHRASN
8   SERASPGLYSERVALSERLEUGLUGLUTYR
9   GLUASPLEUILEILELYSSERLEUGLNLYS
10   ALAGLYILEARGVALGLULYSGLNSERLEU
11   VALPHE

Entity 2, CALCIUM ION_1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: Tcb2-C, [U-98% 13C; U-98% 15N], 0.75 mM; TRIS 25 mM; potassium chloride 50 mM; magnesium chloride 5 mM; EGTA 1 mM; calcium chloride 5 mM

sample_2: Tcb2-C, [U-98% 13C; U-98% 15N], 0.75 mM; TRIS 25 mM; potassium chloride 50 mM; magnesium chloride 5 mM; EGTA 1 mM; calcium chloride 5 mM

sample_3: Tcb2-C, [U-98% 15N], 0.75 mM; TRIS 25 mM; potassium chloride 50 mM; magnesium chloride 5 mM; EGTA 1 mM; calcium chloride 5 mM

sample_4: Tcb2-C 1.1 mM; TRIS 25 mM; potassium chloride 50 mM; magnesium chloride 5 mM; EGTA 1 mM; calcium chloride 5 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HMQCsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HACACOsample_2isotropicsample_conditions_1
2D (HB)CB(CGCD)HDsample_2isotropicsample_conditions_1
2D (HB)CB(CGCDCE)HEsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_3isotropicsample_conditions_1
2D 1H-1H COSYsample_4isotropicsample_conditions_1
2D 1H-1H TOCSYsample_4isotropicsample_conditions_1
2D 1H-1H NOESYsample_4isotropicsample_conditions_1
Heteronuclear NOE ratiosample_1isotropicsample_conditions_1
T1 experimentssample_1isotropicsample_conditions_1
T2 experimentssample_1isotropicsample_conditions_1

Software:

CccpNmr_Analysis, CCPN - chemical shift assignment, data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ, Varian - collection

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

NCBI P09226.2 XP_001007754.3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks