BMRB Entry 26723

Name: NMR chemical shift assignments for the C-terminal domain of Tetrahymena Tcb2 in the absence of calcium
Published: 2016-07-14

Click here to enlarge.

PDB ID: 2nco
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26723
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

NMR chemical shift assignments for the C-terminal domain of Tetrahymena Tcb2 in the absence of calcium

Deposition date:

2015-12-28

Original release date:

2016-07-14

Authors:

Kilpatrick, Adina; Gurrola, Theodore; Fowler, C. Andrew

Citation:

Citation: Kilpatrick, Adina; Gurrola, Theodore; Sterner, Robert; Sleister, Heidi; Honts, Jerry; Fowler, C. Andrew. "Backbone and side-chain chemical shift assignments for the C-terminal domain of Tcb2, a cytoskeletal calcium-binding protein from Tetrahymena thermophila" Biomol. NMR Assign. 10, 281-285 (2016).
PubMed: 27155947

Assembly members:

Assembly members:
Tcb2-C, polymer, 102 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Tetrahymena thermophila Taxonomy ID: 5911 Superkingdom: Eukaryota Kingdom: not available Genus/species: Tetrahymena thermophila

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pJ411: T7 promoter, inducible

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Tcb2-C: SSKPKYNPEVEAKLDVARRL FKRYDKDGSGQLQDDEIAGL LKDTYAEMGMSNFTPTKEDV KIWLQMADTNSDGSVSLEEY EDLIIKSLQKAGIRVEKQSL VF

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
heteronucl_NOEs
- heteronuclear_noe_list_1
heteronucl_T1_relaxation
- heteronuclear_T1_list_1
heteronucl_T2_relaxation
- heteronuclear_T2_list_1

Data type	Count
13C chemical shifts	450
15N chemical shifts	101
1H chemical shifts	677
T1 relaxation values	87
T2 relaxation values	84
heteronuclear NOE values	87

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Tcb2-C	1

Entities:

Entity 1, Tcb2-C 102 residues - Formula weight is not available

C-terminal domain of Tetrahymena calcium-binding protein 2 (Tcb2)

1

SER

LYS

PRO

LYS

TYR

ASN

PRO

GLU

VAL

2

GLU

ALA

LYS

LEU

ASP

VAL

ALA

ARG

LEU

3

PHE

LYS

ARG

TYR

ASP

LYS

ASP

GLY

SER

GLY

4

GLN

LEU

GLN

ASP

GLU

ILE

ALA

GLY

LEU

5

LEU

LYS

ASP

THR

TYR

ALA

GLU

MET

GLY

MET

6

SER

ASN

PHE

THR

PRO

THR

LYS

GLU

ASP

VAL

7

LYS

ILE

TRP

LEU

GLN

MET

ALA

ASP

THR

ASN

8

SER

ASP

GLY

SER

VAL

SER

LEU

GLU

TYR

9

GLU

ASP

LEU

ILE

LYS

SER

LEU

GLN

LYS

10

ALA

GLY

ILE

ARG

VAL

GLU

LYS

GLN

SER

LEU

11

VAL

PHE

Samples:

sample_1: Tcb2-C, [U-98% 13C; U-98% 15N], 0.75 mM; TRIS 25 mM; potassium chloride 50 mM; magnesium chloride 5 mM; EGTA 1 mM

sample_2: Tcb2-C, [U-98% 13C; U-98% 15N], 0.75 mM; TRIS 25 mM; potassium chloride 50 mM; magnesium chloride 5 mM; EGTA 1 mM

sample_3: Tcb2-C, [U-98% 15N], 0.75 mM; TRIS 25 mM; potassium chloride 50 mM; magnesium chloride 5 mM; EGTA 1 mM

sample_4: Tcb2-C 1.1 mM; TRIS 25 mM; potassium chloride 50 mM; magnesium chloride 5 mM; EGTA 1 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-13C HMQC	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_3	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_4	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_4	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_4	isotropic	sample_conditions_1
2D (HB)CB(CGCD)HD	sample_2	isotropic	sample_conditions_1
2D (HB)CB(CGCDCE)HE	sample_2	isotropic	sample_conditions_1
Heteronuclear NOE ratio	sample_1	isotropic	sample_conditions_1
T1 experiments	sample_1	isotropic	sample_conditions_1
T2 experiments	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr_Analysis, CCPN - chemical shift assignment, data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ, Varian - collection

NMR spectrometers:

Varian INOVA 600 MHz