BMRB Entry 26722

Name: 1H, 15N, 13C backbone shift assignments of PhyR protein from Erythrobacter litoralis
Published: 2016-09-01

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26722

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N, 13C backbone shift assignments of PhyR protein from Erythrobacter litoralis

Deposition date:

2015-12-14

Original release date:

2016-09-01

Authors:

Correa, Fernando; Gardner, Kevin

Citation:

Citation: Correa, Fernando; Gardner, Kevin. "Basis of Mutual Domain Inhibition in a Bacterial Response Regulator" Cell Chem. Biol. 23, 945-954 (2016).
PubMed: 27524295

Assembly members:

Assembly members:
ELI10215, polymer, 270 residues, Formula weight is not available

Natural source:

Natural source: Common Name: a-proteobacteria Taxonomy ID: 39960 Superkingdom: Bacteria Kingdom: not available Genus/species: Erythrobacter litoralis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHis - parallel

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ELI10215: GAMGMSASQKIAANLPYLRR YARALTGSQQTGDTFVRATL EAAIADESLKQDVSEGRVPL YKAFNALWSSSYLEVEGDDG GDVVSAKEAGAGDRLKAVTP LNRQALLLTTLEDFSVEDAA EIMGLAPADVEGLVREAVAE IDRESSTNVLIIEDEPLISM QLEDLVRSLGHDIAGTAATR TQAQEAVAKEKPGLVLADIQ LADGSSGIDAVEDILGQFDV PVIFITAYPERLLTGDRPEP TYLVTKPFQESTVRTTISQA LFFQNSPTAV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	497
15N chemical shifts	252
1H chemical shifts	257

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ELI10215	1

Entities:

Entity 1, ELI10215 270 residues - Formula weight is not available

Residues 1-4 (GAMG) are cloning artifacts derived from the expression vector

1	GLY	ALA	MET	GLY	MET	SER	ALA	SER	GLN	LYS
2	ILE	ALA	ALA	ASN	LEU	PRO	TYR	LEU	ARG	ARG
3	TYR	ALA	ARG	ALA	LEU	THR	GLY	SER	GLN	GLN
4	THR	GLY	ASP	THR	PHE	VAL	ARG	ALA	THR	LEU
5	GLU	ALA	ALA	ILE	ALA	ASP	GLU	SER	LEU	LYS
6	GLN	ASP	VAL	SER	GLU	GLY	ARG	VAL	PRO	LEU
7	TYR	LYS	ALA	PHE	ASN	ALA	LEU	TRP	SER	SER
8	SER	TYR	LEU	GLU	VAL	GLU	GLY	ASP	ASP	GLY
9	GLY	ASP	VAL	VAL	SER	ALA	LYS	GLU	ALA	GLY
10	ALA	GLY	ASP	ARG	LEU	LYS	ALA	VAL	THR	PRO
11	LEU	ASN	ARG	GLN	ALA	LEU	LEU	LEU	THR	THR
12	LEU	GLU	ASP	PHE	SER	VAL	GLU	ASP	ALA	ALA
13	GLU	ILE	MET	GLY	LEU	ALA	PRO	ALA	ASP	VAL
14	GLU	GLY	LEU	VAL	ARG	GLU	ALA	VAL	ALA	GLU
15	ILE	ASP	ARG	GLU	SER	SER	THR	ASN	VAL	LEU
16	ILE	ILE	GLU	ASP	GLU	PRO	LEU	ILE	SER	MET
17	GLN	LEU	GLU	ASP	LEU	VAL	ARG	SER	LEU	GLY
18	HIS	ASP	ILE	ALA	GLY	THR	ALA	ALA	THR	ARG
19	THR	GLN	ALA	GLN	GLU	ALA	VAL	ALA	LYS	GLU
20	LYS	PRO	GLY	LEU	VAL	LEU	ALA	ASP	ILE	GLN
21	LEU	ALA	ASP	GLY	SER	SER	GLY	ILE	ASP	ALA
22	VAL	GLU	ASP	ILE	LEU	GLY	GLN	PHE	ASP	VAL
23	PRO	VAL	ILE	PHE	ILE	THR	ALA	TYR	PRO	GLU
24	ARG	LEU	LEU	THR	GLY	ASP	ARG	PRO	GLU	PRO
25	THR	TYR	LEU	VAL	THR	LYS	PRO	PHE	GLN	GLU
26	SER	THR	VAL	ARG	THR	THR	ILE	SER	GLN	ALA
27	LEU	PHE	PHE	GLN	ASN	SER	PRO	THR	ALA	VAL

Samples:

sample_1: ELI10215, [U-13C; U-15N; U-2H], 0.5 – 1 mM; TRIS 10 mM; sodium chloride 50 mM; H2O 90%; D2O 10%

sample_2: ELI10215, [U-99% 13C; U-99% 15N], 0.5 – 1 mM; TRIS 10 mM; sodium chloride 50 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.05 M; pH: 7; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CB	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-15N TROSY HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N TROSY HSQC	sample_2	isotropic	sample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Varian INOVA 800 MHz
Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	GLY	ALA	MET	GLY	MET	SER	ALA	SER	GLN	LYS
2	ILE	ALA	ALA	ASN	LEU	PRO	TYR	LEU	ARG	ARG
3	TYR	ALA	ARG	ALA	LEU	THR	GLY	SER	GLN	GLN
4	THR	GLY	ASP	THR	PHE	VAL	ARG	ALA	THR	LEU
5	GLU	ALA	ALA	ILE	ALA	ASP	GLU	SER	LEU	LYS
6	GLN	ASP	VAL	SER	GLU	GLY	ARG	VAL	PRO	LEU
7	TYR	LYS	ALA	PHE	ASN	ALA	LEU	TRP	SER	SER
8	SER	TYR	LEU	GLU	VAL	GLU	GLY	ASP	ASP	GLY
9	GLY	ASP	VAL	VAL	SER	ALA	LYS	GLU	ALA	GLY
10	ALA	GLY	ASP	ARG	LEU	LYS	ALA	VAL	THR	PRO
11	LEU	ASN	ARG	GLN	ALA	LEU	LEU	LEU	THR	THR
12	LEU	GLU	ASP	PHE	SER	VAL	GLU	ASP	ALA	ALA
13	GLU	ILE	MET	GLY	LEU	ALA	PRO	ALA	ASP	VAL
14	GLU	GLY	LEU	VAL	ARG	GLU	ALA	VAL	ALA	GLU
15	ILE	ASP	ARG	GLU	SER	SER	THR	ASN	VAL	LEU
16	ILE	ILE	GLU	ASP	GLU	PRO	LEU	ILE	SER	MET
17	GLN	LEU	GLU	ASP	LEU	VAL	ARG	SER	LEU	GLY
18	HIS	ASP	ILE	ALA	GLY	THR	ALA	ALA	THR	ARG
19	THR	GLN	ALA	GLN	GLU	ALA	VAL	ALA	LYS	GLU
20	LYS	PRO	GLY	LEU	VAL	LEU	ALA	ASP	ILE	GLN
21	LEU	ALA	ASP	GLY	SER	SER	GLY	ILE	ASP	ALA
22	VAL	GLU	ASP	ILE	LEU	GLY	GLN	PHE	ASP	VAL
23	PRO	VAL	ILE	PHE	ILE	THR	ALA	TYR	PRO	GLU
24	ARG	LEU	LEU	THR	GLY	ASP	ARG	PRO	GLU	PRO
25	THR	TYR	LEU	VAL	THR	LYS	PRO	PHE	GLN	GLU
26	SER	THR	VAL	ARG	THR	THR	ILE	SER	GLN	ALA
27	LEU	PHE	PHE	GLN	ASN	SER	PRO	THR	ALA	VAL

1	GLY	ALA	MET	GLY	MET	SER	ALA	SER	GLN	LYS
2	ILE	ALA	ALA	ASN	LEU	PRO	TYR	LEU	ARG	ARG
3	TYR	ALA	ARG	ALA	LEU	THR	GLY	SER	GLN	GLN
4	THR	GLY	ASP	THR	PHE	VAL	ARG	ALA	THR	LEU
5	GLU	ALA	ALA	ILE	ALA	ASP	GLU	SER	LEU	LYS
6	GLN	ASP	VAL	SER	GLU	GLY	ARG	VAL	PRO	LEU
7	TYR	LYS	ALA	PHE	ASN	ALA	LEU	TRP	SER	SER
8	SER	TYR	LEU	GLU	VAL	GLU	GLY	ASP	ASP	GLY
9	GLY	ASP	VAL	VAL	SER	ALA	LYS	GLU	ALA	GLY
10	ALA	GLY	ASP	ARG	LEU	LYS	ALA	VAL	THR	PRO
11	LEU	ASN	ARG	GLN	ALA	LEU	LEU	LEU	THR	THR
12	LEU	GLU	ASP	PHE	SER	VAL	GLU	ASP	ALA	ALA
13	GLU	ILE	MET	GLY	LEU	ALA	PRO	ALA	ASP	VAL
14	GLU	GLY	LEU	VAL	ARG	GLU	ALA	VAL	ALA	GLU
15	ILE	ASP	ARG	GLU	SER	SER	THR	ASN	VAL	LEU
16	ILE	ILE	GLU	ASP	GLU	PRO	LEU	ILE	SER	MET
17	GLN	LEU	GLU	ASP	LEU	VAL	ARG	SER	LEU	GLY
18	HIS	ASP	ILE	ALA	GLY	THR	ALA	ALA	THR	ARG
19	THR	GLN	ALA	GLN	GLU	ALA	VAL	ALA	LYS	GLU
20	LYS	PRO	GLY	LEU	VAL	LEU	ALA	ASP	ILE	GLN
21	LEU	ALA	ASP	GLY	SER	SER	GLY	ILE	ASP	ALA
22	VAL	GLU	ASP	ILE	LEU	GLY	GLN	PHE	ASP	VAL
23	PRO	VAL	ILE	PHE	ILE	THR	ALA	TYR	PRO	GLU
24	ARG	LEU	LEU	THR	GLY	ASP	ARG	PRO	GLU	PRO
25	THR	TYR	LEU	VAL	THR	LYS	PRO	PHE	GLN	GLU
26	SER	THR	VAL	ARG	THR	THR	ILE	SER	GLN	ALA
27	LEU	PHE	PHE	GLN	ASN	SER	PRO	THR	ALA	VAL

1	GLY	ALA	MET	GLY	MET	SER	ALA	SER	GLN	LYS
2	ILE	ALA	ALA	ASN	LEU	PRO	TYR	LEU	ARG	ARG
3	TYR	ALA	ARG	ALA	LEU	THR	GLY	SER	GLN	GLN
4	THR	GLY	ASP	THR	PHE	VAL	ARG	ALA	THR	LEU
5	GLU	ALA	ALA	ILE	ALA	ASP	GLU	SER	LEU	LYS
6	GLN	ASP	VAL	SER	GLU	GLY	ARG	VAL	PRO	LEU
7	TYR	LYS	ALA	PHE	ASN	ALA	LEU	TRP	SER	SER
8	SER	TYR	LEU	GLU	VAL	GLU	GLY	ASP	ASP	GLY
9	GLY	ASP	VAL	VAL	SER	ALA	LYS	GLU	ALA	GLY
10	ALA	GLY	ASP	ARG	LEU	LYS	ALA	VAL	THR	PRO
11	LEU	ASN	ARG	GLN	ALA	LEU	LEU	LEU	THR	THR
12	LEU	GLU	ASP	PHE	SER	VAL	GLU	ASP	ALA	ALA
13	GLU	ILE	MET	GLY	LEU	ALA	PRO	ALA	ASP	VAL
14	GLU	GLY	LEU	VAL	ARG	GLU	ALA	VAL	ALA	GLU
15	ILE	ASP	ARG	GLU	SER	SER	THR	ASN	VAL	LEU
16	ILE	ILE	GLU	ASP	GLU	PRO	LEU	ILE	SER	MET
17	GLN	LEU	GLU	ASP	LEU	VAL	ARG	SER	LEU	GLY
18	HIS	ASP	ILE	ALA	GLY	THR	ALA	ALA	THR	ARG
19	THR	GLN	ALA	GLN	GLU	ALA	VAL	ALA	LYS	GLU
20	LYS	PRO	GLY	LEU	VAL	LEU	ALA	ASP	ILE	GLN
21	LEU	ALA	ASP	GLY	SER	SER	GLY	ILE	ASP	ALA
22	VAL	GLU	ASP	ILE	LEU	GLY	GLN	PHE	ASP	VAL
23	PRO	VAL	ILE	PHE	ILE	THR	ALA	TYR	PRO	GLU
24	ARG	LEU	LEU	THR	GLY	ASP	ARG	PRO	GLU	PRO
25	THR	TYR	LEU	VAL	THR	LYS	PRO	PHE	GLN	GLU
26	SER	THR	VAL	ARG	THR	THR	ILE	SER	GLN	ALA
27	LEU	PHE	PHE	GLN	ASN	SER	PRO	THR	ALA	VAL