BMRB Entry 26715

Name: beta-endorphin
Published: 2016-07-14

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PDB ID: 6tub
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26715
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

beta-endorphin

Deposition date:

2015-12-07

Original release date:

2016-07-14

Authors:

Seuring, Carolin; Gath, Julia; Verasdonck, Joeri; Nespovitaya, Nadezhda; Cadalbert, Riccardo; Rivier, Jean; Boeckmann, Anja; Meier, Beat; Riek, Roland

Citation:

Citation: Seuring, Carolin; Gath, Julia; Verasdonck, Joeri; Nespovitaya, Nadezhda; Cadalbert, Riccardo; Rivier, Jean; Boeckmann, Anja; Meier, Beat; Riek, Roland. "Solid-state NMR sequential assignment of the beta-endorphin peptide in its amyloid form" Biomol. NMR Assign. 10, 259-268 (2016).
PubMed: 27165576

Assembly members:

Assembly members:
beta-endorphin, polymer, 31 residues, Formula weight is not available

Natural source:

Natural source: Common Name: enterobacteria Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta-endorphin: YGGFMTSEKSQTPLVTLFKN AIIKNAYKKGE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	146
15N chemical shifts	32
1H chemical shifts	52

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	beta_endorphin	1

Entities:

Entity 1, beta_endorphin 31 residues - Formula weight is not available

1

TYR

GLY

PHE

MET

THR

SER

GLU

LYS

SER

2

GLN

THR

PRO

LEU

VAL

THR

LEU

PHE

LYS

ASN

3

ALA

ILE

LYS

ASN

ALA

TYR

LYS

GLY

4

GLU

Samples:

sample_1: beta-endorphin, [U-100% 13C; U-100% 15N], 25 mg; H2O 10%; D2O 90%

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 20ms DARR	sample_1	isotropic	sample_conditions_1
2D NCA	sample_1	isotropic	sample_conditions_1
3D NCACB	sample_1	isotropic	sample_conditions_1
3D CCC	sample_1	isotropic	sample_conditions_1
3D CANCO	sample_1	isotropic	sample_conditions_1
3D NCOCA	sample_1	isotropic	sample_conditions_1
3D NCACX	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 850 MHz