BMRB Entry 26709

Name: Backbone Assignment of Human linear diubiquitin
Published: 2016-01-14

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26709

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone Assignment of Human linear diubiquitin

Deposition date:

2015-11-26

Original release date:

2016-01-14

Authors:

Halander, Jenny; Messias, Ana; Geerlof, Arie; Sattler, Michael

Citation:

Citation: Vincendeau, Michelle; Kamyar, Hadian; Messias, Ana; Brenke, Jara; Halander, Jenny; Griesbach, Richard; Greczmiel, Ute; Bertossi, Arianna; Stehle, Ralf; Nagel, Daniel; Demski, Kathrin; Velvarska, Hana; Niessing, Dierk; Geerlof, Arie; Sattler, Michael; Krappmann, Daniel. "Inhibition of Canonical NF-kB Signaling by a Small Molecule Targeting NEMO-Ubiquitin Interaction" Sci. Rep. 6, 18934-18934 (2016).
PubMed: 26740240

Assembly members:

Assembly members:
linear_diubiquitin, polymer, 162 residues, 18220.8 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pASK-IBA-3plus

Entity Sequences (FASTA):

Entity Sequences (FASTA):
linear_diubiquitin: MASHHHHHHGAQIFVKTLTG KTITLEVEPSDTIENVKAKI QDKEGIPPDQQRLIFAGKQL EDGRTLSDYNIQKESTLHLV LRLRGGMQIFVKTLTGKTIT LEVEPSDTIENVKAKIQDKE GIPPDQQRLIFAGKQLEDGR TLSDYNIQKESTLHLVLRLR GG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	255
15N chemical shifts	132
1H chemical shifts	132

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	linear diubiquitin	1

Entities:

Entity 1, linear diubiquitin 162 residues - 18220.8 Da.

Residues 1-11 are non-native residues part of the N-terminal affinity tag.

1

MET

ALA

SER

HIS

GLY

2

ALA

GLN

ILE

PHE

VAL

LYS

THR

LEU

THR

GLY

3

LYS

THR

ILE

THR

LEU

GLU

VAL

GLU

PRO

SER

4

ASP

THR

ILE

GLU

ASN

VAL

LYS

ALA

LYS

ILE

5

GLN

ASP

LYS

GLU

GLY

ILE

PRO

ASP

GLN

6

GLN

ARG

LEU

ILE

PHE

ALA

GLY

LYS

GLN

LEU

7

GLU

ASP

GLY

ARG

THR

LEU

SER

ASP

TYR

ASN

8

ILE

GLN

LYS

GLU

SER

THR

LEU

HIS

LEU

VAL

9

LEU

ARG

LEU

ARG

GLY

MET

GLN

ILE

PHE

10

VAL

LYS

THR

LEU

THR

GLY

LYS

THR

ILE

THR

11

LEU

GLU

VAL

GLU

PRO

SER

ASP

THR

ILE

GLU

12

ASN

VAL

LYS

ALA

LYS

ILE

GLN

ASP

LYS

GLU

13

GLY

ILE

PRO

ASP

GLN

ARG

LEU

ILE

14

PHE

ALA

GLY

LYS

GLN

LEU

GLU

ASP

GLY

ARG

15

THR

LEU

SER

ASP

TYR

ASN

ILE

GLN

LYS

GLU

16

SER

THR

LEU

HIS

LEU

VAL

LEU

ARG

LEU

ARG

17

GLY

Samples:

sample_1: linear diubiquitin, [U-100% 13C; U-100% 15N], 0.9 mM; D2O 10%; Tris-HCl, [U-2H], 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 0.07 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AV III 800 MHz

UNIPROT	P0CG47
PDB	2W9N 3AXC
AlphaFold	Q9UPK7