BMRB Entry 26708

Title:
Backbone Assignment of Human NEMO residues 258-350
Deposition date:
2015-11-24
Original release date:
2016-01-14
Authors:
Halander, Jenny; Messias, Ana; Geerlof, Arie; Sattler, Michael
Citation:

Citation: Vincendeau, Michelle; Kamyar, Hadian; Messias, Ana; Brenke, Jara; Halander, Jenny; Griesbach, Richard; Greczmiel, Ute; Bertossi, Arianna; Stehle, Ralf; Nagel, Daniel; Demski, Kathrin; Velvarska, Hana; Niessing, Dierk; Geerlof, Arie; Sattler, Michael; Krappmann, Daniel. "Inhibition of Canonical NF-kB Signaling by a Small Molecule Targeting NEMO-Ubiquitin Interaction"  Sci. Rep. 6, 18934-18934 (2016).
PubMed: 26740240

Assembly members:

Assembly members:
NEMO, polymer, 95 residues, 11011.5 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM11

Data sets:
Data typeCount
13C chemical shifts148
15N chemical shifts75
1H chemical shifts75

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NEMO 11
2NEMO 21

Entities:

Entity 1, NEMO 1 95 residues - 11011.5 Da.

Residues 1-2 are non-native residues that remain after cleavage of the N-terminal affinity tag.

1   GLYALAMETGLNLEUGLUASPLEULYSGLN
2   GLNLEUGLNGLNALAGLUGLUALALEUVAL
3   ALALYSGLNGLUVALILEASPLYSLEULYS
4   GLUGLUALAGLUGLNHISLYSILEVALMET
5   GLUTHRVALPROVALLEULYSALAGLNALA
6   ASPILETYRLYSALAASPPHEGLNALAGLU
7   ARGGLNALAARGGLULYSLEUALAGLULYS
8   LYSGLULEULEUGLNGLUGLNLEUGLUGLN
9   LEUGLNARGGLUTYRSERLYSLEULYSALA
10   SERCYSGLNGLUSER

Samples:

sample_1: NEMO, [U-13C; U-15N; U-2H], 1.2 mM; D2O 10%; H2O 90%; sodium phosphate 50 mM; sodium NaCl 50 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AV III 800 MHz

Related Database Links:

UNIPROT Q9Y6K9
PDB
AlphaFold Q7Z7F1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks