BMRB Entry 26705

Name: Backbone chemical shifts of the oligomerization inhibited mutant of the CASKIN2 SAM domain tandem
Published: 2016-06-29

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26705

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone chemical shifts of the oligomerization inhibited mutant of the CASKIN2 SAM domain tandem

Deposition date:

2015-11-09

Original release date:

2016-06-29

Authors:

Donaldson, Logan

Citation:

Citation: Smirnova, Ekaterina; Kwan, Jamie; Siu, Ryan; Gao, Xin; Zoid, Georg; Demeler, Borries; Saridakis, Vivian; Donaldson, Logan. "A new mode of SAM domain mediated oligomerization observed in the CASKIN2 neuronal scaffolding protein" Cell Commun. Signal 14, 17-17 (2016).
PubMed: 27549312

Assembly members:

Assembly members:
CASKIN2, polymer, 171 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CASKIN2: gsshhhhhhssglvprgsME QLLEGKDAQAIHNWLSEFQL EGYTAHFLQAGYDVPTISRM TPEDLTAIGVTKPDHREKIA SEIAQLSIAEWLPSYIPTDL LEWLCALGLPQYHKQLVSSG YDSMGLVADLTWEELQEIGV NKLGHQKKLMLGVKRLAELR RGLLQGEALSE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	360
15N chemical shifts	114
1H chemical shifts	114

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SAMSAM monomer	1

Entities:

Entity 1, SAMSAM monomer 171 residues - Formula weight is not available

1

GLY

SER

HIS

SER

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

MET

GLU

3

GLN

LEU

GLU

GLY

LYS

ASP

ALA

GLN

ALA

4

ILE

HIS

ASN

TRP

LEU

SER

GLU

PHE

GLN

LEU

5

GLU

GLY

TYR

THR

ALA

HIS

PHE

LEU

GLN

ALA

6

GLY

TYR

ASP

VAL

PRO

THR

ILE

SER

ARG

MET

7

THR

PRO

GLU

ASP

LEU

THR

ALA

ILE

GLY

VAL

8

THR

LYS

PRO

ASP

HIS

ARG

GLU

LYS

ILE

ALA

9

SER

GLU

ILE

ALA

GLN

LEU

SER

ILE

ALA

GLU

10

TRP

LEU

PRO

SER

TYR

ILE

PRO

THR

ASP

LEU

11

LEU

GLU

TRP

LEU

CYS

ALA

LEU

GLY

LEU

PRO

12

GLN

TYR

HIS

LYS

GLN

LEU

VAL

SER

GLY

13

TYR

ASP

SER

MET

GLY

LEU

VAL

ALA

ASP

LEU

14

THR

TRP

GLU

LEU

GLN

GLU

ILE

GLY

VAL

15

ASN

LYS

LEU

GLY

HIS

GLN

LYS

LEU

MET

16

LEU

GLY

VAL

LYS

ARG

LEU

ALA

GLU

LEU

ARG

17

ARG

GLY

LEU

GLN

GLY

GLU

ALA

LEU

SER

18

GLU

Samples:

sample_1: CASKIN2, [U-99% 13C; U-99% 15N], 0.8 mM; potassium chloride 20 mM; sodium azide 0.05 % w/v; sodium chloride 150 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.8; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

Analysis v2.4, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks