BMRB Entry 26701

Name: T-STAR KH domain
Published: 2016-01-06

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26701

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

T-STAR KH domain

Deposition date:

2015-11-04

Original release date:

2016-01-06

Authors:

Feracci, Mikael; Dominguez, Cyril

Citation:

Citation: Feracci, Mikael; Foot, Jaelle; Grellscheid, Sushma; Danilenko, Marina; Stehle, Ralf; Gonchar, Oksana; Kang, Hyun-Seo; Dalgliesh, Caroline; Meyer, Helge; Liu, Yilei; Lahat, Albert; Sattler, Michael; Eperon, Ian; Elliott, David; Dominguez, Cyril. "Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68" Nat. Commun. 7, 10355-10355 (2016).
PubMed: 26758068

Assembly members:

Assembly members:
T_STAR, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pLEICS-03

Entity Sequences (FASTA):

Entity Sequences (FASTA):
T_STAR: GAINKNMKLGQKVLIPVKQF PKFNFVGKLLGPRGNSLKRL QEETLTKMSILGKGSMRDKA KEEELRKSGEAKYFHLNDDL HVLIEVFAPPAEAYARMGHA LEEIKKFLIPDYN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	101
1H chemical shifts	101

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	KH domain	1

Entities:

Entity 1, KH domain 113 residues - Formula weight is not available

residues 1 and 2 are non-native amino acids from the tag after Tev cleavage

1

GLY

ALA

ILE

ASN

LYS

ASN

MET

LYS

LEU

GLY

2

GLN

LYS

VAL

LEU

ILE

PRO

VAL

LYS

GLN

PHE

3

PRO

LYS

PHE

ASN

PHE

VAL

GLY

LYS

LEU

4

GLY

PRO

ARG

GLY

ASN

SER

LEU

LYS

ARG

LEU

5

GLN

GLU

THR

LEU

THR

LYS

MET

SER

ILE

6

LEU

GLY

LYS

GLY

SER

MET

ARG

ASP

LYS

ALA

7

LYS

GLU

LEU

ARG

LYS

SER

GLY

GLU

8

ALA

LYS

TYR

PHE

HIS

LEU

ASN

ASP

LEU

9

HIS

VAL

LEU

ILE

GLU

VAL

PHE

ALA

PRO

10

ALA

GLU

ALA

TYR

ALA

ARG

MET

GLY

HIS

ALA

11

LEU

GLU

ILE

LYS

PHE

LEU

ILE

PRO

12

ASP

TYR

ASN

Samples:

sample_1: T_STAR, [U-15N], 0.5 ± 0.1 mM; T_STAR, [U-13C; U-15N], 1 ± 0.1 mM; H2O 93%; D2O 7%; sodium phosphate 20 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.2; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY v3.110, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

UNP	O75525
AlphaFold	Q9UPA8