BMRB Entry 26692

Name: Solid State NMR sequential assignment of Amyloid b(1-42) fibrils
Published: 2016-05-24

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PDB ID: 2nao
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR26692
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solid State NMR sequential assignment of Amyloid b(1-42) fibrils

Deposition date:

2015-10-23

Original release date:

2016-05-24

Authors:

Ravotti, Francesco; Waelti, Marielle; Guentert, Peter; Meier, Beat; Riek, Roland; Boeckmann, Anja

Citation:

Citation: Ravotti, Francesco; Waelti, Marielle; Guentert, Peter; Meier, Beat; Riek, Roland; Boeckmann, Anja. "Solid-State NMR sequential assignment of Amyloid-b (1-42) fibrils" Biomol. NMR Assign. 10, 269-276 (2016).
PubMed: 27165577

Assembly members:

Assembly members:
Amyloid_Beta, polymer, 42 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Amyloid_Beta: DAEFRHDSGYEVHHQKLVFF AEDVGSNKGAIIGLMVGGVV IA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	169
15N chemical shifts	44

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Amyloid Beta fibril	1

Entities:

Entity 1, Amyloid Beta fibril 42 residues - Formula weight is not available

1

ASP

ALA

GLU

PHE

ARG

HIS

ASP

SER

GLY

TYR

2

GLU

VAL

HIS

GLN

LYS

LEU

VAL

PHE

3

ALA

GLU

ASP

VAL

GLY

SER

ASN

LYS

GLY

ALA

4

ILE

GLY

LEU

MET

VAL

GLY

VAL

5

ILE

ALA

Samples:

sample_1: Amyloid Beta, [U-13C], 20 mg

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
NCACX	sample_1	isotropic	sample_conditions_1
NCOCX	sample_1	isotropic	sample_conditions_1
CANCO	sample_1	isotropic	sample_conditions_1
NCACB	sample_1	isotropic	sample_conditions_1
CCC	sample_1	isotropic	sample_conditions_1
NCA	sample_1	isotropic	sample_conditions_1
NCO	sample_1	isotropic	sample_conditions_1
DARR	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin, CCPN - chemical shift assignment, data analysis, processing

NMR spectrometers:

Bruker Avance 850 MHz