BMRB Entry 26684

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26684

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Title:
Chica 410-478
Deposition date:
2015-10-07
Original release date:
2015-12-17
Authors:
Nyarko, Afua; Clark, Sarah; Barbar, Elisar
Citation:

Citation: Clark, Sarah; Nyarko, Afua; Lohr, Frank; Karplus, P. Andrew; Barbar, Elisar. "The anchored flexibility model in LC8 motif recognition: Insights from the Chica complex"  Biochemistry 55, 199-209 (2015).
PubMed: 26652654

Assembly members:

Assembly members:
Chica, polymer, 615 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Chica: MARACLIQRLPIKRDCTPVF VRGLSSPSAAMALLSEGLDE VPAACLSPCGPPNPTELFSE SRRLALEELVAGGPEAFAAF LRRERLARFLNPDEVHAILR AAERPGEEGAAAAAAAEDSF GSSHDCSSGTYFPEQSDLEP PLLELGWPAFYQGAYRGATR VETHFQPRGAGEGGPYGCKD ALRQQLRSAREVIAVVMDVF TDIDIFRDLQEICRKQGVAV YILLDQALLSQFLDMCMDLK VHPEQEKLMTVRTITGNIYY ARSGTKIIGKVHEKFTLIDG IRVATGSYSFTWTDGKLNSS NLVILSGQVVEHFDLEFRIL YAQSKPISPKLLSHFQSSNK FDHLTNRKPQSKELTLGNLL RMRLARLSSTPRKADLDPEM PAEGKAERKPHDCESSTVSE EDYFSSHRDELQSRKAIDAA TQTEPGEEMPGLSVSEVGTQ TSITTACAGTQTAVITRIAS SQTTIWSRSTTTQTDMDENI LFPRGTQSTEGSPVSKMSVS RSSSLKSSSSVSSQGSVASS TGSPASIRTTDFHNPGYPKY LGTPHLELYLSDSLRNLNKE RQFHFAGIRSRLNHMLAMLS RRTLFTENHLGLHSGNFSRV NLLAVRDVALYPSYQ

Data sets:
Data typeCount
13C chemical shifts199
15N chemical shifts67
1H chemical shifts67

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Chica1

Entities:

Entity 1, Chica 615 residues - Formula weight is not available

1   METALAARGALACYSLEUILEGLNARGLEU
2   PROILELYSARGASPCYSTHRPROVALPHE
3   VALARGGLYLEUSERSERPROSERALAALA
4   METALALEULEUSERGLUGLYLEUASPGLU
5   VALPROALAALACYSLEUSERPROCYSGLY
6   PROPROASNPROTHRGLULEUPHESERGLU
7   SERARGARGLEUALALEUGLUGLULEUVAL
8   ALAGLYGLYPROGLUALAPHEALAALAPHE
9   LEUARGARGGLUARGLEUALAARGPHELEU
10   ASNPROASPGLUVALHISALAILELEUARG
11   ALAALAGLUARGPROGLYGLUGLUGLYALA
12   ALAALAALAALAALAALAGLUASPSERPHE
13   GLYSERSERHISASPCYSSERSERGLYTHR
14   TYRPHEPROGLUGLNSERASPLEUGLUPRO
15   PROLEULEUGLULEUGLYTRPPROALAPHE
16   TYRGLNGLYALATYRARGGLYALATHRARG
17   VALGLUTHRHISPHEGLNPROARGGLYALA
18   GLYGLUGLYGLYPROTYRGLYCYSLYSASP
19   ALALEUARGGLNGLNLEUARGSERALAARG
20   GLUVALILEALAVALVALMETASPVALPHE
21   THRASPILEASPILEPHEARGASPLEUGLN
22   GLUILECYSARGLYSGLNGLYVALALAVAL
23   TYRILELEULEUASPGLNALALEULEUSER
24   GLNPHELEUASPMETCYSMETASPLEULYS
25   VALHISPROGLUGLNGLULYSLEUMETTHR
26   VALARGTHRILETHRGLYASNILETYRTYR
27   ALAARGSERGLYTHRLYSILEILEGLYLYS
28   VALHISGLULYSPHETHRLEUILEASPGLY
29   ILEARGVALALATHRGLYSERTYRSERPHE
30   THRTRPTHRASPGLYLYSLEUASNSERSER
31   ASNLEUVALILELEUSERGLYGLNVALVAL
32   GLUHISPHEASPLEUGLUPHEARGILELEU
33   TYRALAGLNSERLYSPROILESERPROLYS
34   LEULEUSERHISPHEGLNSERSERASNLYS
35   PHEASPHISLEUTHRASNARGLYSPROGLN
36   SERLYSGLULEUTHRLEUGLYASNLEULEU
37   ARGMETARGLEUALAARGLEUSERSERTHR
38   PROARGLYSALAASPLEUASPPROGLUMET
39   PROALAGLUGLYLYSALAGLUARGLYSPRO
40   HISASPCYSGLUSERSERTHRVALSERGLU
41   GLUASPTYRPHESERSERHISARGASPGLU
42   LEUGLNSERARGLYSALAILEASPALAALA
43   THRGLNTHRGLUPROGLYGLUGLUMETPRO
44   GLYLEUSERVALSERGLUVALGLYTHRGLN
45   THRSERILETHRTHRALACYSALAGLYTHR
46   GLNTHRALAVALILETHRARGILEALASER
47   SERGLNTHRTHRILETRPSERARGSERTHR
48   THRTHRGLNTHRASPMETASPGLUASNILE
49   LEUPHEPROARGGLYTHRGLNSERTHRGLU
50   GLYSERPROVALSERLYSMETSERVALSER
51   ARGSERSERSERLEULYSSERSERSERSER
52   VALSERSERGLNGLYSERVALALASERSER
53   THRGLYSERPROALASERILEARGTHRTHR
54   ASPPHEHISASNPROGLYTYRPROLYSTYR
55   LEUGLYTHRPROHISLEUGLULEUTYRLEU
56   SERASPSERLEUARGASNLEUASNLYSGLU
57   ARGGLNPHEHISPHEALAGLYILEARGSER
58   ARGLEUASNHISMETLEUALAMETLEUSER
59   ARGARGTHRLEUPHETHRGLUASNHISLEU
60   GLYLEUHISSERGLYASNPHESERARGVAL
61   ASNLEULEUALAVALARGASPVALALALEU
62   TYRPROSERTYRGLN

Samples:

sample_1: Chica, [U-99% 13C; U-99% 15N], 250 uM; DSS 1 mM; sodium azide 1 mM; sodium phosphate 10 mM; sodium chloride 10 mM; arginine/glutamate 50 mM

sample_conditions_1: ionic strength: 0.01 M; pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5, Bruker Biospin - collection

SPARKY, Shen and Bax - chemical shift assignment, data analysis, processing

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks