BMRB Entry 26674

Name: Structural basis for the inhibition of voltage-gated sodium channels with conotoxin-muOxi-GVIIJ
Published: 2016-02-01

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PDB ID: 2n8h
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26674
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structural basis for the inhibition of voltage-gated sodium channels with conotoxin-muOxi-GVIIJ

Deposition date:

2015-09-30

Original release date:

2016-02-01

Authors:

Green, Brad; Chhabra, Sandeep; Norton, Raymond

Citation:

Citation: Green, Brad; Gajewiak, Joanna; Chhabra, Sandeep; Skalicky, Jack; Zhang, Min-Min; Bulaj, Grzegorz; Olivera, Baldomero; Yoshikami, Doju; Norton, Raymond. "Structural basis for the inhibition of voltage-gated sodium channels with conotoxin-muOxi-GVIIJ" J. Biol. Chem. 291, 7205-7220 (2016).
PubMed: 26817840

Assembly members:

Assembly members:
GVIIJ_C24S, polymer, 35 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GVIIJ_C24S: GWCGDXGATCGKLRLYCCSG FCDSYTKTCKDKSSA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	63
15N chemical shifts	31
1H chemical shifts	202

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GVIIJ[C24S]	1

Entities:

Entity 1, GVIIJ[C24S] 35 residues - Formula weight is not available

1

GLY

TRP

CYS

GLY

ASP

HYP

GLY

ALA

THR

CYS

2

GLY

LYS

LEU

ARG

LEU

TYR

CYS

SER

GLY

3

PHE

CYS

ASP

SER

TYR

THR

LYS

THR

CYS

LYS

4

ASP

LYS

SER

ALA

Samples:

sample_1: GVIIJ_C24S 1.4 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 10 mM; pH: 3.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr v2.1.5, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Avance 600 MHz
Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks