BMRB Entry 26656

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26656

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Title:
Backbone 1H, 13C, 15N Chemical Shift Assignments for Full-length HIV-1 Transactivator of Transcription
Deposition date:
2015-09-11
Original release date:
2016-09-09
Authors:
To, Vu; O'Neil, Joe
Citation:

Citation: To, Vu; Dzananovic, Edis; McKenna, Sean; O'Neil, Joe. "The Dynamic Landscape of the Full-Length HIV-1 Transactivator of Transcription"  Biochemistry 55, 1314-1325 (2016).
PubMed: 26866386

Assembly members:

Assembly members:
Tat101, polymer, 121 residues, 13670.4717 Da.

Natural source:

Natural source:   Common Name: HIV-1   Taxonomy ID: 11676   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lentivirus HIV-1

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Tat101: MGSSHHHHHHSSGLVPRGSH MEPVDPRLEPWKHPGSQPKT ACTNCYCKKCCFHCQVCFIT KALGISYGRKKRRQRRRPPQ GSQTHQVSLSKQPASQPRGD PTGPKESKKKVERETETDPV D

Data sets:
Data typeCount
13C chemical shifts326
15N chemical shifts105
1H chemical shifts215

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Tat1011

Entities:

Entity 1, Tat101 121 residues - 13670.4717 Da.

Full-length HIV-1 Transactivator of transcription. This construct has a his-tag and thrombin digestion site on the N-terminal, accounting for the first 20 amino acids.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METGLUPROVALASPPROARGLEUGLUPRO
4   TRPLYSHISPROGLYSERGLNPROLYSTHR
5   ALACYSTHRASNCYSTYRCYSLYSLYSCYS
6   CYSPHEHISCYSGLNVALCYSPHEILETHR
7   LYSALALEUGLYILESERTYRGLYARGLYS
8   LYSARGARGGLNARGARGARGPROPROGLN
9   GLYSERGLNTHRHISGLNVALSERLEUSER
10   LYSGLNPROALASERGLNPROARGGLYASP
11   PROTHRGLYPROLYSGLUSERLYSLYSLYS
12   VALGLUARGGLUTHRGLUTHRASPPROVAL
13   ASP

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks