BMRB Entry 26649

Name: 1H, 13C, 15N chemical shift assignments of initiation factor 1 from Pseudomonas aeruginosa
Published: 2016-08-22

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PDB ID: 2n78
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR26649
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, 15N chemical shift assignments of initiation factor 1 from Pseudomonas aeruginosa

Deposition date:

2015-09-04

Original release date:

2016-08-22

Authors:

Zhang, Yonghong

Citation:

Citation: Bernal, Alejandra; Hu, Yanmei; Palmer, Stephanie; Silva, Aaron; Bullard, James; Zhang, Yonghong. "1H, 13C and 15N resonance assignments and secondary structure analysis of translation initiation factor 1 from Pseudomonas aeruginosa" Biomol NMR Assign. 10, 249-252 (2016).
PubMed: 26983940

Assembly members:

Assembly members:
Initiation_Factor_IF-1, polymer, 80 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Initiation_Factor_IF-1: MSKEDSFEMEGTVVDTLPNT MFRVELENGHVVTAHISGKM RKNYIRILTGDKVRVELTPY DLSKGRITYRARLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	269
15N chemical shifts	70
1H chemical shifts	460

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	IF1	1

Entities:

Entity 1, IF1 80 residues - Formula weight is not available

1	MET	SER	LYS	GLU	ASP	SER	PHE	GLU	MET	GLU
2	GLY	THR	VAL	VAL	ASP	THR	LEU	PRO	ASN	THR
3	MET	PHE	ARG	VAL	GLU	LEU	GLU	ASN	GLY	HIS
4	VAL	VAL	THR	ALA	HIS	ILE	SER	GLY	LYS	MET
5	ARG	LYS	ASN	TYR	ILE	ARG	ILE	LEU	THR	GLY
6	ASP	LYS	VAL	ARG	VAL	GLU	LEU	THR	PRO	TYR
7	ASP	LEU	SER	LYS	GLY	ARG	ILE	THR	TYR	ARG
8	ALA	ARG	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

Samples:

sample_1: Initiation Factor IF-1, [U-100% 13C; U-100% 15N], 1 mM; KPO4 25 mM; KCl 100 mM

sample_conditions_1: ionic strength: 0.125 M; pH: 5.1; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	MET	SER	LYS	GLU	ASP	SER	PHE	GLU	MET	GLU
2	GLY	THR	VAL	VAL	ASP	THR	LEU	PRO	ASN	THR
3	MET	PHE	ARG	VAL	GLU	LEU	GLU	ASN	GLY	HIS
4	VAL	VAL	THR	ALA	HIS	ILE	SER	GLY	LYS	MET
5	ARG	LYS	ASN	TYR	ILE	ARG	ILE	LEU	THR	GLY
6	ASP	LYS	VAL	ARG	VAL	GLU	LEU	THR	PRO	TYR
7	ASP	LEU	SER	LYS	GLY	ARG	ILE	THR	TYR	ARG
8	ALA	ARG	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	SER	LYS	GLU	ASP	SER	PHE	GLU	MET	GLU
2	GLY	THR	VAL	VAL	ASP	THR	LEU	PRO	ASN	THR
3	MET	PHE	ARG	VAL	GLU	LEU	GLU	ASN	GLY	HIS
4	VAL	VAL	THR	ALA	HIS	ILE	SER	GLY	LYS	MET
5	ARG	LYS	ASN	TYR	ILE	ARG	ILE	LEU	THR	GLY
6	ASP	LYS	VAL	ARG	VAL	GLU	LEU	THR	PRO	TYR
7	ASP	LEU	SER	LYS	GLY	ARG	ILE	THR	TYR	ARG
8	ALA	ARG	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	SER	LYS	GLU	ASP	SER	PHE	GLU	MET	GLU
2	GLY	THR	VAL	VAL	ASP	THR	LEU	PRO	ASN	THR
3	MET	PHE	ARG	VAL	GLU	LEU	GLU	ASN	GLY	HIS
4	VAL	VAL	THR	ALA	HIS	ILE	SER	GLY	LYS	MET
5	ARG	LYS	ASN	TYR	ILE	ARG	ILE	LEU	THR	GLY
6	ASP	LYS	VAL	ARG	VAL	GLU	LEU	THR	PRO	TYR
7	ASP	LEU	SER	LYS	GLY	ARG	ILE	THR	TYR	ARG
8	ALA	ARG	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS