BMRB Entry 26647

Name: NFkB p65 DNA-binding domain
Published: 2016-09-02

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26647

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NFkB p65 DNA-binding domain

Deposition date:

2015-09-02

Original release date:

2016-09-02

Authors:

Mukherjee, Sulakshana; Quintas, Pedro; Borin, Brendan

Citation:

Citation: Mukherjee, Sulakshana; Borin, Brendan; Quintas, Pedro; Dyson, Jane. "NMR characterization of a 72 kDa transcription factor using differential isotopic labeling" Protein Sci. 25, 597-604 (2016).
PubMed: 26647230

Assembly members:

Assembly members:
p65DBD, polymer, 173 residues, Formula weight is not available

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
p65DBD: PYVEIIEQPKQRGMRFRYKC EGRSAGSIPGERSTDTTKTH PTIKINGYTGPGTVRISLVT KDPPHRPHPHELVGKDCRDG YYEADLCPDRSIHSFQNLGI QCVKKRDLEQAISQRIQTNN NPFHVPIEEQRGDYDLNAVR LCFQVTVRDPAGRPLLLTPV LSHPIFDNRAPNT

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	296
15N chemical shifts	151
1H chemical shifts	499

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	p65 DBD	1

Entities:

Entity 1, p65 DBD 173 residues - Formula weight is not available

1

PRO

TYR

VAL

GLU

ILE

GLU

GLN

PRO

LYS

2

GLN

ARG

GLY

MET

ARG

PHE

ARG

TYR

LYS

CYS

3

GLU

GLY

ARG

SER

ALA

GLY

SER

ILE

PRO

GLY

4

GLU

ARG

SER

THR

ASP

THR

LYS

THR

HIS

5

PRO

THR

ILE

LYS

ILE

ASN

GLY

TYR

THR

GLY

6

PRO

GLY

THR

VAL

ARG

ILE

SER

LEU

VAL

THR

7

LYS

ASP

PRO

HIS

ARG

PRO

HIS

PRO

HIS

8

GLU

LEU

VAL

GLY

LYS

ASP

CYS

ARG

ASP

GLY

9

TYR

GLU

ALA

ASP

LEU

CYS

PRO

ASP

ARG

10

SER

ILE

HIS

SER

PHE

GLN

ASN

LEU

GLY

ILE

11

GLN

CYS

VAL

LYS

ARG

ASP

LEU

GLU

GLN

12

ALA

ILE

SER

GLN

ARG

ILE

GLN

THR

ASN

13

ASN

PRO

PHE

HIS

VAL

PRO

ILE

GLU

GLN

14

ARG

GLY

ASP

TYR

ASP

LEU

ASN

ALA

VAL

ARG

15

LEU

CYS

PHE

GLN

VAL

THR

VAL

ARG

ASP

PRO

16

ALA

GLY

ARG

PRO

LEU

THR

PRO

VAL

17

LEU

SER

HIS

PRO

ILE

PHE

ASP

ASN

ARG

ALA

18

PRO

ASN

THR

Samples:

sample_1: p65DBD, [U-100% 13C; U-100% 15N], 0.3 ± 0.1 mM; TRIS, [U-100% 2H], 20 mM; sodium chloride 50 mM; DTT, [U-100% 2H], 2 mM; D2O, [U-100% 2H], 10%; H2O 90%

sample_conditions_1: ionic strength: 0.05 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

Bruker DRX 600 MHz
Bruker Avance 750 MHz