BMRB Entry 26645

Name: Backbone 1H, 15N, 13C NMR assignment of the 518-627 fragment of the androgen receptor encompassing N-terminal and DNA Binding domains
Published: 2016-07-15

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26645

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 15N, 13C NMR assignment of the 518-627 fragment of the androgen receptor encompassing N-terminal and DNA Binding domains

Deposition date:

2015-09-01

Original release date:

2016-07-15

Authors:

Kieffer, Bruno; Meyer, Sandra; Perez Escriva, Pau; Wang, Ying-Hui

Citation:

Citation: Meyer, Sandra; Wang, Ying-Hui; Perez Escriva, Pau; Kieffer, Bruno. "Backbone 1H, 15N, 13C NMR assignment of the 518-627 fragment of the androgen receptor encompassing N-terminal and DNA binding domains" J. Biomol. NMR 10, 175-178 (2016).
PubMed: 26732902

Assembly members:

Assembly members:
AR_NTD_DBD_518-627, polymer, 114 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM41

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AR_NTD_DBD_518-627: GAMGCVKSEMGPWMDSYSGP YGDMRLETARDHVLPIDYYF PPQKTCLICGDEASGCHYGA LTCGSCKVFFKRAAEGKQKY LCASRNDCTIDKFRRKNCPS CRLRKCYEAGMTLG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	308
15N chemical shifts	104
1H chemical shifts	105

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AR	1
2	ZN	2

Entities:

Entity 1, AR 114 residues - Formula weight is not available

1

GLY

ALA

MET

GLY

CYS

VAL

LYS

SER

GLU

MET

2

GLY

PRO

TRP

MET

ASP

SER

TYR

SER

GLY

PRO

3

TYR

GLY

ASP

MET

ARG

LEU

GLU

THR

ALA

ARG

4

ASP

HIS

VAL

LEU

PRO

ILE

ASP

TYR

PHE

5

PRO

GLN

LYS

THR

CYS

LEU

ILE

CYS

GLY

6

ASP

GLU

ALA

SER

GLY

CYS

HIS

TYR

GLY

ALA

7

LEU

THR

CYS

GLY

SER

CYS

LYS

VAL

PHE

8

LYS

ARG

ALA

GLU

GLY

LYS

GLN

LYS

TYR

9

LEU

CYS

ALA

SER

ARG

ASN

ASP

CYS

THR

ILE

10

ASP

LYS

PHE

ARG

LYS

ASN

CYS

PRO

SER

11

CYS

ARG

LEU

ARG

LYS

CYS

TYR

GLU

ALA

GLY

12

MET

THR

LEU

GLY

Entity 2, ZN - 65.409 Da.

1

ZN

Samples:

sample_1: AR NTD DBD 518-627, [U-99% 13C; U-99% 15N], 260 ± 40 uM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 180 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CCPNMR, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz

UNP	E7EVX6_HUMAN
AlphaFold	Q9UD95