BMRB Entry 26643

Name: Backbone 1H, 13C, and 15N chemical shift assignments for the N-WASP-GBD Nck complex
Published: 2015-09-23

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26643

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N chemical shift assignments for the N-WASP-GBD Nck complex

Deposition date:

2015-08-30

Original release date:

2015-09-23

Authors:

Wu, Qiong; Okrut, Julia; Taunton, Jack

Citation:

Citation: Okrut, Julia; Prakash, Sumit; Wu, Qiong; Kelly, Mark; Taunton, Jack. "Allosteric N-WASP activation by an inter-SH3 domain linker in Nck" .

Assembly members:

Assembly members:
GBD-Nck, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: modified pet24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GBD-Nck: GHMTKADIGTPSNFQHIGHV GWDPNTGFDLNNLDPELKNL FDMCGISEAQLKDRETSKVI YDFIEKTGGVEAVKNELRRQ GGSGGSARKASIVKNLKDTL GIGKVKRK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	264
15N chemical shifts	88
1H chemical shifts	136

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GBD-Nck	1

Entities:

Entity 1, GBD-Nck 108 residues - Formula weight is not available

Residue 1-3 are cloning artifacts. Residues 4-80 correspond to N-WASP aa 196-272. Residues 81-86 constitute a GGSGGS linker. Residues 87-108 correspond to Nck1 aa 62-83.

1

GLY

HIS

MET

THR

LYS

ALA

ASP

ILE

GLY

THR

2

PRO

SER

ASN

PHE

GLN

HIS

ILE

GLY

HIS

VAL

3

GLY

TRP

ASP

PRO

ASN

THR

GLY

PHE

ASP

LEU

4

ASN

LEU

ASP

PRO

GLU

LEU

LYS

ASN

LEU

5

PHE

ASP

MET

CYS

GLY

ILE

SER

GLU

ALA

GLN

6

LEU

LYS

ASP

ARG

GLU

THR

SER

LYS

VAL

ILE

7

TYR

ASP

PHE

ILE

GLU

LYS

THR

GLY

VAL

8

GLU

ALA

VAL

LYS

ASN

GLU

LEU

ARG

GLN

9

GLY

SER

GLY

SER

ALA

ARG

LYS

ALA

10

SER

ILE

VAL

LYS

ASN

LEU

LYS

ASP

THR

LEU

11

GLY

ILE

GLY

LYS

VAL

LYS

ARG

LYS

Samples:

sample_1: GBD-Nck, [U-98% 13C; U-98% 15N], 550 uM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Zhengrong and Bax - processing

VNMRJ v4.2, Varian - collection

NMR spectrometers:

Agilent DD2 600 MHz

BMRB	18165
PDB	2LNH
DBJ	BAA11082 BAA21534 BAE25143 BAE25639 BAE28201
EMBL	CAC69994 CAH89371 CAH91010 CAL26602
GB	AAH52955 AAH55045 AAI51608 AAQ96857 AAV38348
REF	NP_001103835 NP_001128797 NP_001253726 NP_003932 NP_082735
SP	O00401 O08816 Q91YD9 Q95107
TPG	DAA30422
AlphaFold	O00401 O08816 Q91YD9 Q95107