BMRB Entry 26642

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26642

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Title:
Backbone 1H, 13C and 15N Chemical Shift Assignments for the Plakin Repeat Domain of Envoplakin
Deposition date:
2015-08-28
Original release date:
2015-12-17
Authors:
Overduin, Michael; Jeeves, Mark; Fogl, Claudia; Al-Jassar, Caezar; Chidgey, Martyn
Citation:

Citation: Jeeves, Mark; Fogl, Claudia; Al-Jassar, Caezar; Chidgey, Martyn; Overduin, Michael. "Sequence-specific 1H, 13C and 15N backbone resonance assignments of the plakin repeat domain of human envoplakin"  Biomol. NMR Assignments 10, 167-170 (2016).
PubMed: 26590577

Assembly members:

Assembly members:
Envoplakin_PRD, polymer, 201 residues, 21999.2 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pProEx-HTc

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Envoplakin_PRD: GAMGIRNSDDSFPIAGIYDT TTDNKCSIKTAVAKNMLDPI TGQKLLEAQAATGGIVDLLS RERYSVHKAMERGLIENTST QRLLNAQKAFTGIEDPVTKK RLSVGEAVQKGWMPRESVLP HLQVQHLTGGLIDPKRTGRI PIQQALLSGMISEELAQLLQ DESSYEKDLTDPISKERLSY KEAMGRCRKDPLSGLLLLPA A

Data sets:
Data typeCount
13C chemical shifts562
15N chemical shifts184
1H chemical shifts184

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Envoplakin PRD1

Entities:

Entity 1, Envoplakin PRD 201 residues - 21999.2 Da.

Residues 1-8 are the remnants of a non-native His affinity tag. This is the plakin repeat domain of envoplakin.

1   GLYALAMETGLYILEARGASNSERASPASP
2   SERPHEPROILEALAGLYILETYRASPTHR
3   THRTHRASPASNLYSCYSSERILELYSTHR
4   ALAVALALALYSASNMETLEUASPPROILE
5   THRGLYGLNLYSLEULEUGLUALAGLNALA
6   ALATHRGLYGLYILEVALASPLEULEUSER
7   ARGGLUARGTYRSERVALHISLYSALAMET
8   GLUARGGLYLEUILEGLUASNTHRSERTHR
9   GLNARGLEULEUASNALAGLNLYSALAPHE
10   THRGLYILEGLUASPPROVALTHRLYSLYS
11   ARGLEUSERVALGLYGLUALAVALGLNLYS
12   GLYTRPMETPROARGGLUSERVALLEUPRO
13   HISLEUGLNVALGLNHISLEUTHRGLYGLY
14   LEUILEASPPROLYSARGTHRGLYARGILE
15   PROILEGLNGLNALALEULEUSERGLYMET
16   ILESERGLUGLULEUALAGLNLEULEUGLN
17   ASPGLUSERSERTYRGLULYSASPLEUTHR
18   ASPPROILESERLYSGLUARGLEUSERTYR
19   LYSGLUALAMETGLYARGCYSARGLYSASP
20   PROLEUSERGLYLEULEULEULEUPROALA
21   ALA

Samples:

sample_1: Envoplakin PRD, [U-99% 13C; U-99% 15N], 800 uM; D2O 10%; NaCl 50 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPNMR_Analysis, CCPN - peak picking

NMR spectrometers:

  • Varian INOVA 900 MHz
  • Varian INOVA 600 MHz

Related Database Links:

NCBI NP_001979.2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks