BMRB Entry 26641

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26641

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Title:
Backbone assignments for E. coli MurD
Deposition date:
2015-08-27
Original release date:
2015-12-17
Authors:
Ogura, Kenji; Kumeta, Hiroyuki; Yokochi, Masashi; Inagaki, Fuyuhiko
Citation:

Citation: Saio, Tomohide; Ogura, Kenji; Kumeta, Hiroyuki; Kobashigawa, Yoshihiro; Shimizu, Kazumi; Yokochi, Masashi; Kodama, Kota; Yamaguchi, Hiroto; Tsujishita, Hideki; Inagaki, Fuyuhiko. "Ligand-driven conformational changes of MurD visualized by paramagnetic NMR"  Sci. Rep. ., .-. (2015).
PubMed: 2015

Assembly members:

Assembly members:
MurD, polymer, 441 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: enterobacteria   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGBHPS

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MurD: HMGSADYQGKNVVIIGLGLT GLSCVDFFLARGVTPRVMDT RMTPPGLDKLPEAVERHTGS LNDEWLMAADLIVASPGIAL AHPSLSAAADAGIEIVGDIE LFCREAQAPIVAITGSNGKS TVTTLVGEMAKAAGVNVGVG GNIGLPALMLLDDECELYVL ELSSFQLETTSSLQAVAATI LNVTEDHMDRYPFGLQQYRA AKLRIYENAKVCVVNADDAL TMPIRGADERCVSFGVNMGD YHLNHQQGETWLRVKGEKVL NVKEMKLSGQHNYTNALAAL ALADAAGLPRASSLKALTTF TGLPHRFEVVLEHNGVRWIN DSKATNVGSTEAALNGLHVD GTLHLLLGGDGKSADFSPLA RYLNGDNVRLYCFGRDGAQL AALRPEVAEQTETMEQAMRL LAPRVQPGDMVLLSPACASL DQFKNFEQRGNEFARLAKEL G

Data sets:
Data typeCount
15N chemical shifts371
1H chemical shifts371

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MurD monomer1

Entities:

Entity 1, MurD monomer 441 residues - Formula weight is not available

"HMGS" at the N-terminus is cloning artifact. Ala right after "HMGS" is the first residue.

1   HISMETGLYSERALAASPTYRGLNGLYLYS
2   ASNVALVALILEILEGLYLEUGLYLEUTHR
3   GLYLEUSERCYSVALASPPHEPHELEUALA
4   ARGGLYVALTHRPROARGVALMETASPTHR
5   ARGMETTHRPROPROGLYLEUASPLYSLEU
6   PROGLUALAVALGLUARGHISTHRGLYSER
7   LEUASNASPGLUTRPLEUMETALAALAASP
8   LEUILEVALALASERPROGLYILEALALEU
9   ALAHISPROSERLEUSERALAALAALAASP
10   ALAGLYILEGLUILEVALGLYASPILEGLU
11   LEUPHECYSARGGLUALAGLNALAPROILE
12   VALALAILETHRGLYSERASNGLYLYSSER
13   THRVALTHRTHRLEUVALGLYGLUMETALA
14   LYSALAALAGLYVALASNVALGLYVALGLY
15   GLYASNILEGLYLEUPROALALEUMETLEU
16   LEUASPASPGLUCYSGLULEUTYRVALLEU
17   GLULEUSERSERPHEGLNLEUGLUTHRTHR
18   SERSERLEUGLNALAVALALAALATHRILE
19   LEUASNVALTHRGLUASPHISMETASPARG
20   TYRPROPHEGLYLEUGLNGLNTYRARGALA
21   ALALYSLEUARGILETYRGLUASNALALYS
22   VALCYSVALVALASNALAASPASPALALEU
23   THRMETPROILEARGGLYALAASPGLUARG
24   CYSVALSERPHEGLYVALASNMETGLYASP
25   TYRHISLEUASNHISGLNGLNGLYGLUTHR
26   TRPLEUARGVALLYSGLYGLULYSVALLEU
27   ASNVALLYSGLUMETLYSLEUSERGLYGLN
28   HISASNTYRTHRASNALALEUALAALALEU
29   ALALEUALAASPALAALAGLYLEUPROARG
30   ALASERSERLEULYSALALEUTHRTHRPHE
31   THRGLYLEUPROHISARGPHEGLUVALVAL
32   LEUGLUHISASNGLYVALARGTRPILEASN
33   ASPSERLYSALATHRASNVALGLYSERTHR
34   GLUALAALALEUASNGLYLEUHISVALASP
35   GLYTHRLEUHISLEULEULEUGLYGLYASP
36   GLYLYSSERALAASPPHESERPROLEUALA
37   ARGTYRLEUASNGLYASPASNVALARGLEU
38   TYRCYSPHEGLYARGASPGLYALAGLNLEU
39   ALAALALEUARGPROGLUVALALAGLUGLN
40   THRGLUTHRMETGLUGLNALAMETARGLEU
41   LEUALAPROARGVALGLNPROGLYASPMET
42   VALLEULEUSERPROALACYSALASERLEU
43   ASPGLNPHELYSASNPHEGLUGLNARGGLY
44   ASNGLUPHEALAARGLEUALALYSGLULEU
45   GLY

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks