BMRB Entry 26639

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26639

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Title:
1H, 13C and 15N Chemical Shift Assignments for human CBP-ID4
Deposition date:
2015-08-21
Original release date:
2016-01-22
Authors:
Piai, Alessandro; Calcada, Eduardo; Tarenzi, Thomas; Del Grande, Alessandro; Tompa, Peter; Felli, Isabella; Pierattelli, Roberta
Citation:

Citation: Piai, Alessandro; Calcada, Eduardo; Tarenzi, Thomas; Del Grande, Alessandro; Varadi, M.; Tompa, Peter; Felli, Isabella; Pierattelli, Roberta. "Just a Flexible Linker? The Structural and Dynamic Properties of CBP-ID4 Revealed by NMR Spectroscopy"  Biophys. J. 110, 372-381 (2016).
PubMed: 26789760

Assembly members:

Assembly members:
CBP-ID4, polymer, 207 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a-CBP-ID4

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CBP-ID4: MQQQIQHRLQQAQLMRRRMA TMNTRNVPQQSLPSPTSAPP GTPTQQPSTPQTPQPPAQPQ PSPVSMSPAGFPSVARTQPP TTVSTGKPTSQVPAPPPPAQ PPPAAVEAARQIEREAQQQQ HLYRVNINNSMPPGRTGMGT PGSQMAPVSLNVPRPNQVSG PVMPSMPPGQWQQAPLPQQQ PMPGLPRPVISMQAQAAVAG PRMPSVQ

Data sets:
Data typeCount
13C chemical shifts604
15N chemical shifts205
1H chemical shifts160

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CBP-ID41

Entities:

Entity 1, CBP-ID4 207 residues - Formula weight is not available

1   METGLNGLNGLNILEGLNHISARGLEUGLN
2   GLNALAGLNLEUMETARGARGARGMETALA
3   THRMETASNTHRARGASNVALPROGLNGLN
4   SERLEUPROSERPROTHRSERALAPROPRO
5   GLYTHRPROTHRGLNGLNPROSERTHRPRO
6   GLNTHRPROGLNPROPROALAGLNPROGLN
7   PROSERPROVALSERMETSERPROALAGLY
8   PHEPROSERVALALAARGTHRGLNPROPRO
9   THRTHRVALSERTHRGLYLYSPROTHRSER
10   GLNVALPROALAPROPROPROPROALAGLN
11   PROPROPROALAALAVALGLUALAALAARG
12   GLNILEGLUARGGLUALAGLNGLNGLNGLN
13   HISLEUTYRARGVALASNILEASNASNSER
14   METPROPROGLYARGTHRGLYMETGLYTHR
15   PROGLYSERGLNMETALAPROVALSERLEU
16   ASNVALPROARGPROASNGLNVALSERGLY
17   PROVALMETPROSERMETPROPROGLYGLN
18   TRPGLNGLNALAPROLEUPROGLNGLNGLN
19   PROMETPROGLYLEUPROARGPROVALILE
20   SERMETGLNALAGLNALAALAVALALAGLY
21   PROARGMETPROSERVALGLN

Samples:

sample_1: CBP-ID4, [U-100% 13C; U-100% 15N], 0.6 mM; potassium phosphate 20 mM; potassium chloride 100 mM; H2O 90%; D2O, [U-99% 2H], 10%

sample_conditions_1: pH: 6.5; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D CON-IPAPsample_1isotropicsample_conditions_1
3D (H)CBCACON-IPAPsample_1isotropicsample_conditions_1
3D (H)CBCANCO-IPAPsample_1isotropicsample_conditions_1
4D (HCA)CON(CA)CON-IPAPsample_1isotropicsample_conditions_1
4D (HN)CON(CA)CON-IPAPsample_1isotropicsample_conditions_1
2D BEST-TROSYsample_1isotropicsample_conditions_1
3D BT-HNCOsample_1isotropicsample_conditions_1
3D BT-HN(CA)COsample_1isotropicsample_conditions_1
3D BT-HNCACBsample_1isotropicsample_conditions_1
3D BT-HN(CO)CACBsample_1isotropicsample_conditions_1
3D BT-(H)N(COCA)NHsample_1isotropicsample_conditions_1
3D (H)N(CA)NNHsample_1isotropicsample_conditions_1
5D BT-(H)NCO(CAN)CONHsample_1isotropicsample_conditions_1
5D BT-HN(COCAN)CONHsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 900 MHz
  • Bruker Avance III 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks