BMRB Entry 26638

Name: Backbone and side chain chemical shift assignments of apolipophorin III from Galleria mellonella
Published: 2015-12-17

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26638

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and side chain chemical shift assignments of apolipophorin III from Galleria mellonella

Deposition date:

2015-08-20

Original release date:

2015-12-17

Authors:

Crowhurst, Karin; Weers, Paul

Citation:

Citation: Crowhurst, Karin; Horn, James; Weers, Paul. "Backbone and side chain chemical shift assignments of apolipophorin III from Galleria mellonella" Biomol. NMR Assign. 10, 143-147 (2016).
PubMed: 26493308

Assembly members:

Assembly members:
apolipophorin_III, polymer, 163 residues, Formula weight is not available

Natural source:

Natural source: Common Name: greater wax moth Taxonomy ID: 7137 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Galleria mellonella

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
apolipophorin_III: DASTPLQDLEKHAAEFQKTF SEQLNAFTNSKDTKEFNTAL KEGSDSVLQQLNALASSLQK ALNDANGKAKEALEQTRTNL ERTAEELRRAHPDVERQAGA LRDRLQTAVQATVQETQKLA KTVGANLEETNKKLAPQIKS AYDDFVKQAQEVQKKLHEAA SKQ

Data sets:

assigned_chemical_shifts
- ApoLP-3_chem_shift_list

Data type	Count
13C chemical shifts	625
15N chemical shifts	185
1H chemical shifts	828

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	apolipophorin III	1

Entities:

Entity 1, apolipophorin III 163 residues - Formula weight is not available

1

ASP

ALA

SER

THR

PRO

LEU

GLN

ASP

LEU

GLU

2

LYS

HIS

ALA

GLU

PHE

GLN

LYS

THR

PHE

3

SER

GLU

GLN

LEU

ASN

ALA

PHE

THR

ASN

SER

4

LYS

ASP

THR

LYS

GLU

PHE

ASN

THR

ALA

LEU

5

LYS

GLU

GLY

SER

ASP

SER

VAL

LEU

GLN

6

LEU

ASN

ALA

LEU

ALA

SER

LEU

GLN

LYS

7

ALA

LEU

ASN

ASP

ALA

ASN

GLY

LYS

ALA

LYS

8

GLU

ALA

LEU

GLU

GLN

THR

ARG

THR

ASN

LEU

9

GLU

ARG

THR

ALA

GLU

LEU

ARG

ALA

10

HIS

PRO

ASP

VAL

GLU

ARG

GLN

ALA

GLY

ALA

11

LEU

ARG

ASP

ARG

LEU

GLN

THR

ALA

VAL

GLN

12

ALA

THR

VAL

GLN

GLU

THR

GLN

LYS

LEU

ALA

13

LYS

THR

VAL

GLY

ALA

ASN

LEU

GLU

THR

14

ASN

LYS

LEU

ALA

PRO

GLN

ILE

LYS

SER

15

ALA

TYR

ASP

PHE

VAL

LYS

GLN

ALA

GLN

16

GLU

VAL

GLN

LYS

LEU

HIS

GLU

ALA

17

SER

LYS

GLN

Samples:

apolipophorin_III_sample: apolipophorin III, [U-13C; U-15N], 1.2 mM; potassium phosphate 200 mM; sodium azide 2 mM; DSS 0.2 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 0.3 M; pH: 6; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	apolipophorin_III_sample	isotropic	sample_conditions_1
2D 1H-15N HSQC NH2 only	apolipophorin_III_sample	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	apolipophorin_III_sample	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	apolipophorin_III_sample	isotropic	sample_conditions_1
3D CBCA(CO)NH	apolipophorin_III_sample	isotropic	sample_conditions_1
3D C(CO)NH	apolipophorin_III_sample	isotropic	sample_conditions_1
3D HNCO	apolipophorin_III_sample	isotropic	sample_conditions_1
3D HNCACB	apolipophorin_III_sample	isotropic	sample_conditions_1
3D HBHA(CO)NH	apolipophorin_III_sample	isotropic	sample_conditions_1
3D HN(CO)CA	apolipophorin_III_sample	isotropic	sample_conditions_1
3D H(CCO)NH	apolipophorin_III_sample	isotropic	sample_conditions_1
3D HCCH-TOCSY	apolipophorin_III_sample	isotropic	sample_conditions_1
3D 1H-15N NOESY	apolipophorin_III_sample	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	apolipophorin_III_sample	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	apolipophorin_III_sample	isotropic	sample_conditions_1
3D HCACO	apolipophorin_III_sample	isotropic	sample_conditions_1
3D HNHB	apolipophorin_III_sample	isotropic	sample_conditions_1
2D HbCbCgCdHd	apolipophorin_III_sample	isotropic	sample_conditions_1
2D HbCbCgCdCeHe	apolipophorin_III_sample	isotropic	sample_conditions_1

Software:

NMRPipe v8.2, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw v8.2, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView v9.0.0-b114, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Agilent DD2 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks