BMRB Entry 26636

Name: 1H and 15N Chemical Shifts of Intrinsically Disordered Dehydrin ERD14 at Various Temperature and pH Values
Published: 2015-09-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26636

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H and 15N Chemical Shifts of Intrinsically Disordered Dehydrin ERD14 at Various Temperature and pH Values

Deposition date:

2015-08-15

Original release date:

2015-09-15

Authors:

Szalaine Agoston, Bianka

Citation:

Citation: Murvai, Nikoletta; Kalmar, Lajos; Szalaine Agoston, Bianka; Szabo, Beata; Tantos, Agnes; Csikos, Gyorgy; Micsonai, Andras; Kardos, Jozsef; Vertommen, Didier; Nguyen, Phuong; Hristozova, Nevena; Lang, Andras; Kovacs, Denes; Buday, Laszlo; Han, Kyou-Hoon; Perczel, Andras; Tompa, Peter. "Interplay of Structural Disorder and Short Binding Elements in the Cellular Chaperone Function of Plant Dehydrin ERD14" Cells 9, 1856-1856 (2020).
PubMed: 32784707

Assembly members:

Assembly members:
ERD14, polymer, 185 residues, 20786.3 Da.

Natural source:

Natural source: Common Name: thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ERD14: MAEEIKNVPEQEVPKVATEE SSAEVTDRGLFDFLGKKKDE TKPEETPIASEFEQKVHISE PEPEVKHESLLEKLHRSDSS SSSSSEEEGSDGEKRKKKKE KKKPTTEVEVKEEEKKGFME KLKEKLPGHKKPEDGSAVAA APVVVPPPVEEAHPVEKKGI LEKIKEKLPGYHPKTTVEEE KKDKE

Data sets:

assigned_chemical_shifts

Data type	Count
15N chemical shifts	916
1H chemical shifts	916

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ERD14	1

Entities:

Entity 1, ERD14 185 residues - 20786.3 Da.

1

MET

ALA

GLU

ILE

LYS

ASN

VAL

PRO

GLU

2

GLN

GLU

VAL

PRO

LYS

VAL

ALA

THR

GLU

3

SER

ALA

GLU

VAL

THR

ASP

ARG

GLY

LEU

4

PHE

ASP

PHE

LEU

GLY

LYS

ASP

GLU

5

THR

LYS

PRO

GLU

THR

PRO

ILE

ALA

SER

6

GLU

PHE

GLU

GLN

LYS

VAL

HIS

ILE

SER

GLU

7

PRO

GLU

PRO

GLU

VAL

LYS

HIS

GLU

SER

LEU

8

LEU

GLU

LYS

LEU

HIS

ARG

SER

ASP

SER

9

SER

GLU

GLY

SER

10

ASP

GLY

GLU

LYS

ARG

LYS

GLU

11

LYS

PRO

THR

GLU

VAL

GLU

VAL

12

LYS

GLU

LYS

GLY

PHE

MET

GLU

13

LYS

LEU

LYS

GLU

LYS

LEU

PRO

GLY

HIS

LYS

14

LYS

PRO

GLU

ASP

GLY

SER

ALA

VAL

ALA

15

ALA

PRO

VAL

PRO

VAL

GLU

16

GLU

ALA

HIS

PRO

VAL

GLU

LYS

GLY

ILE

17

LEU

GLU

LYS

ILE

LYS

GLU

LYS

LEU

PRO

GLY

18

TYR

HIS

PRO

LYS

THR

VAL

GLU

19

LYS

ASP

LYS

GLU

Samples:

sample_1: ERD14, [U-15N], 1 mM; DSS 1 mM; sodium phosphate 32 mM; potassium phosphate 22 mM; sodium chloride 8.6 mM; calcium chloride 0.1 mM; magnesium sulfate 1 mM; carbenicillin 50 mg/L; glucose.H2O 4 g/L; H2O 90%; D2O 10%

288K-6.58pH: pH: 6.58; pressure: 1 atm; temperature: 288 K

282.5K-6.58pH: pH: 6.58; pressure: 1 atm; temperature: 282.5 K

277K-6.58pH: pH: 6.58; pressure: 1 atm; temperature: 277 K

277K-6.94pH: pH: 6.94; pressure: 1 atm; temperature: 277 K

277K-7.29pH: pH: 7.29; pressure: 1 atm; temperature: 277 K

277K-7.42pH: pH: 7.42; pressure: 1 atm; temperature: 277 K

277K-7.57pH: pH: 7.57; pressure: 1 atm; temperature: 277 K

277K-7.70pH: pH: 7.70; pressure: 1 atm; temperature: 277 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	288K-6.58pH
2D 1H-15N HSQC	sample_1	isotropic	282.5K-6.58pH
2D 1H-15N HSQC	sample_1	isotropic	277K-6.58pH
2D 1H-15N HSQC	sample_1	isotropic	277K-6.94pH
2D 1H-15N HSQC	sample_1	isotropic	277K-7.29pH
2D 1H-15N HSQC	sample_1	isotropic	277K-7.42pH
2D 1H-15N HSQC	sample_1	isotropic	277K-7.57pH
2D 1H-15N HSQC	sample_1	isotropic	277K-7.70pH

Software:

CARA v1.8.4.2, Rochus Keller - chemical shift assignment, peak picking

NMR spectrometers:

Bruker DRX 500 MHz

UNP	P42763
AlphaFold	Q9C5R5 P42763