BMRB Entry 26631

Name: SPOP_MATH_PUC complex
Published: 2016-01-05

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26631

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SPOP_MATH_PUC complex

Deposition date:

2015-08-10

Original release date:

2016-01-05

Authors:

Grace, Christy; Pierce, Wendy; Mittag, Tanja

Citation:

Citation: Pierce, Wendy; Grace, Christy; Lee, Jihun; Nourse, Amanda; Marzahn, Melissa; Watson, Edmond; High, Anthony; Peng, Junmin; Schulman, Brenda; Mittag, Tanja. "Multiple weak linear motifs enhance recruitment and processivity in SPOP-mediated substrate ubiquitination" J. Mol. Biol. 428, 1256-1271 (2016).
PubMed: 26475525

Assembly members:

Assembly members:
MATH-PUC_complex, polymer, 159 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MATH-PUC_complex: GSSGSSGKVVKFSYMWTINN FSFCREEMGEVIKSSTFSSG ANDKLKWCLRVNPKGLDEES KDYLSLYLLLVSCPKSEVRA KFKFSILNAKGEETKAMESQ RAYRFVQGKDWGFKKFIRRD FLLDEANGLLPDDKLTLFCE VSVVQDSVNISGQSGPSSG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	375
15N chemical shifts	128
1H chemical shifts	130

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MATH	1
2	PUC	1

Entities:

Entity 1, MATH 159 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

LYS

VAL

2

LYS

PHE

SER

TYR

MET

TRP

THR

ILE

ASN

3

PHE

SER

PHE

CYS

ARG

GLU

MET

GLY

GLU

4

VAL

ILE

LYS

SER

THR

PHE

SER

GLY

5

ALA

ASN

ASP

LYS

LEU

LYS

TRP

CYS

LEU

ARG

6

VAL

ASN

PRO

LYS

GLY

LEU

ASP

GLU

SER

7

LYS

ASP

TYR

LEU

SER

LEU

TYR

LEU

8

VAL

SER

CYS

PRO

LYS

SER

GLU

VAL

ARG

ALA

9

LYS

PHE

LYS

PHE

SER

ILE

LEU

ASN

ALA

LYS

10

GLY

GLU

THR

LYS

ALA

MET

GLU

SER

GLN

11

ARG

ALA

TYR

ARG

PHE

VAL

GLN

GLY

LYS

ASP

12

TRP

GLY

PHE

LYS

PHE

ILE

ARG

ASP

13

PHE

LEU

ASP

GLU

ALA

ASN

GLY

LEU

14

PRO

ASP

LYS

LEU

THR

LEU

PHE

CYS

GLU

15

VAL

SER

VAL

GLN

ASP

SER

VAL

ASN

ILE

16

SER

GLY

GLN

SER

GLY

PRO

SER

GLY

Samples:

sample_1: MATH, [U-100% 13C; U-100% 15N], 0.5 mM; PUC 2.5 mM; sodium chloride 50.0 mM; potassium chloride 2.7 mM; sodium phosphate 10.0 mM; potassium phosphate 1.76 mM; DTT 5.0 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO; sample_1; sample_conditions_1	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks