BMRB Entry 26625

Title:
PH domain of the Arf GAP ASAP1
Deposition date:
2015-08-06
Original release date:
2015-09-29
Authors:
Li, Yifei; Byrd, Andrew
Citation:

Citation: Jian, Xiaoying; Tang, Wai-Kwan; Zhai, Peng; Roy, Neeladri; Luo, Ruibai; Gruschus, James; Yohe, Marielle; Chen, Pei-Wen; Li, Yifei; Byrd, Andrew; Xia, Di; Randazzo, Paul. "Molecular Basis for Cooperative Binding of Anionic Phospholipids to the PH Domain of the Arf GAP ASAP1"  Structure 23, 1977-1988 (2015).
PubMed: 26365802

Assembly members:

Assembly members:
ASAP1_PH_domain, polymer, 111 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET19B

Data sets:
Data typeCount
13C chemical shifts1
15N chemical shifts97
1H chemical shifts99

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ASAP1 PH domain1

Entities:

Entity 1, ASAP1 PH domain 111 residues - Formula weight is not available

residue numbering begins at 341

1   GLULYSLYSGLYPHELEULEULYSLYSSER
2   ASPGLYILEARGLYSVALTRPGLNARGARG
3   LYSCYSALAVALLYSASNGLYILELEUTHR
4   ILESERHISALATHRSERASNARGGLNPRO
5   ALALYSLEUASNLEULEUTHRCYSGLNVAL
6   LYSPROASNALAGLUASPLYSLYSSERPHE
7   ASPLEUILESERHISASNARGTHRTYRHIS
8   PHEGLNALAGLUASPGLUGLNASPTYRILE
9   ALATRPILESERVALLEUTHRASNSERLYS
10   GLUGLUALALEUTHRMETALAPHEARGGLY
11   GLUGLNSERTHRGLYGLUASNSERLEUGLU
12   ASP

Samples:

sample_1: ASAP1 PH domain, [U-99% 13C; U-99% 15N], 250 uM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks