BMRB Entry 26618

Name: Resonance assignment of DVU2108 that is part of the Orange Protein complex in Desulfovibrio vulgaris Hildenborough
Published: 2015-09-29

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26618

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Resonance assignment of DVU2108 that is part of the Orange Protein complex in Desulfovibrio vulgaris Hildenborough

Deposition date:

2015-07-30

Original release date:

2015-09-29

Authors:

Neca, Ant nio; Soares, Rui; Carepo, Marta; Pauleta, Sofia

Citation:

Citation: Neca, Ant nio; Soares, Rui; Carepo, Marta; Pauleta, Sofia. "Resonance assignment of DVU2108 that is part of the Orange Protein complex in Desulfovibrio vulgaris Hildenborough" Biomol. NMR Assignments 10, 117-120 (2016).
PubMed: 26373427

Assembly members:

Assembly members:
Molecule_1, polymer, 119 residues, 12456.035 Da.

Natural source:

Natural source: Common Name: Desulfovibrio vulgaris str. Hildenborough Taxonomy ID: 882 Superkingdom: Bacteria Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Desulfovibrio vulgaris Hildenborough Vector: pET21c

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Molecule_1: MRIVITAQGNTLDSPLDPRF GRARSFIVCDTETGATHAVD VSANMNLAQGAGIQAAQMAA DAGAEAVITGHVGPKAFTAL NRGHIAVYLCDLATPREALA AFIEGKLRPADTADREGHW

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	476
15N chemical shifts	114
1H chemical shifts	629

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ORP	1

Entities:

Entity 1, ORP 119 residues - 12456.035 Da.

1

MET

ARG

ILE

VAL

ILE

THR

ALA

GLN

GLY

ASN

2

THR

LEU

ASP

SER

PRO

LEU

ASP

PRO

ARG

PHE

3

GLY

ARG

ALA

ARG

SER

PHE

ILE

VAL

CYS

ASP

4

THR

GLU

THR

GLY

ALA

THR

HIS

ALA

VAL

ASP

5

VAL

SER

ALA

ASN

MET

ASN

LEU

ALA

GLN

GLY

6

ALA

GLY

ILE

GLN

ALA

GLN

MET

ALA

7

ASP

ALA

GLY

ALA

GLU

ALA

VAL

ILE

THR

GLY

8

HIS

VAL

GLY

PRO

LYS

ALA

PHE

THR

ALA

LEU

9

ASN

ARG

GLY

HIS

ILE

ALA

VAL

TYR

LEU

CYS

10

ASP

LEU

ALA

THR

PRO

ARG

GLU

ALA

LEU

ALA

11

ALA

PHE

ILE

GLU

GLY

LYS

LEU

ARG

PRO

ALA

12

ASP

THR

ALA

ASP

ARG

GLU

GLY

HIS

TRP

Samples:

15NapoDVU2108: Molecule_1, [U-100% 15N], 1.000 mM; sodium phosphate 20.000 mM; DTT 1.000 mM; NaCl 100.000 mM; sodium azide 0.001 mM; H2O 90%; D2O 10%

15N13CapoDVU2108: Molecule_1, [U-100% 13C; U-100% 15N], 1.0 mM; sodium phosphate 20.0 mM; NaCl 100.0 mM; DTT 1.0 mM; Sodium azide 1.0 mM; H2O 90%; D2O 10%

Temperature: pH: 7.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
15NHSQC	15NapoDVU2108	isotropic	Temperature
HNCO	15N13CapoDVU2108	isotropic	Temperature
HNCACO	15N13CapoDVU2108	isotropic	Temperature
HNHA	15NapoDVU2108	isotropic	Temperature
CBCACONH	15N13CapoDVU2108	isotropic	Temperature
CBCANH	15N13CapoDVU2108	isotropic	Temperature
HBHA(CBCACO)NH	15N13CapoDVU2108	isotropic	Temperature
(H)C(CCO)NH	15N13CapoDVU2108	isotropic	Temperature
H(CCCO)NH	15N13CapoDVU2108	isotropic	Temperature
(H)CCH-TOCSY	15N13CapoDVU2108	isotropic	Temperature
13CHSQC	15N13CapoDVU2108	isotropic	Temperature
13CNOESY	15N13CapoDVU2108	isotropic	Temperature
15NHSQC	15NapoDVU2108	isotropic	Temperature

Software:

CARA v1.8, Rochus Keller, rkeller@nmr.ch - Assignment

CcpNmr_Analysis v2.2, CCPN - Deposit

TopSpin v3.1, Bruker - Processing

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks