BMRB Entry 26617

Name: Backbone Assignments for non-selective ion channel NaK tetramer
Published: 2015-12-18

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26617

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone Assignments for non-selective ion channel NaK tetramer

Deposition date:

2015-07-24

Original release date:

2015-12-18

Authors:

Brettmann, Joshua; Henzler-Wildman, Katherine

Citation:

Citation: Brettmann, Joshua; Urusova, Darya; Tonelli, Marco; Silva, Jonathan; Henzler-Wildman, Katherine. "Role of protein dynamics in ion selectivity and allosteric coupling in the NaK channel" Proc. Natl. Acad. Sci. U.S.A. 112, 15366-15371 (2015).
PubMed: 26621745

Assembly members:

Assembly members:
NaK, polymer, 96 residues, Formula weight is not available

Natural source:

Natural source: Common Name: firmicutes Taxonomy ID: 1396 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus cereus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Pet15B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NaK: GSHMAKDKEFQVLFVLTILT LISGTIFYSTVEGLRPIDAL YFSVVTLTTVGDGNFSPQTD FGKIFTILYIFIGIGLVFGF IHKLAVNVQLPSILSN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	199
15N chemical shifts	77
1H chemical shifts	79

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NaK, chain 1	1
2	NaK, chain 2	1
3	NaK, chain 3	1
4	NaK, chain 4	1

Entities:

Entity 1, NaK, chain 1 96 residues - Formula weight is not available

1

GLY

SER

HIS

MET

ALA

LYS

ASP

LYS

GLU

PHE

2

GLN

VAL

LEU

PHE

VAL

LEU

THR

ILE

LEU

THR

3

LEU

ILE

SER

GLY

THR

ILE

PHE

TYR

SER

THR

4

VAL

GLU

GLY

LEU

ARG

PRO

ILE

ASP

ALA

LEU

5

TYR

PHE

SER

VAL

THR

LEU

THR

VAL

6

GLY

ASP

GLY

ASN

PHE

SER

PRO

GLN

THR

ASP

7

PHE

GLY

LYS

ILE

PHE

THR

ILE

LEU

TYR

ILE

8

PHE

ILE

GLY

ILE

GLY

LEU

VAL

PHE

GLY

PHE

9

ILE

HIS

LYS

LEU

ALA

VAL

ASN

VAL

GLN

LEU

10

PRO

SER

ILE

LEU

SER

ASN

Samples:

NaK_19: NaK, [U-100% 13C; U-100% 15N; U-70% 2H], 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 40 mM; pH: 7; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	NaK_19	isotropic	sample_conditions_1
3D HNCO	NaK_19	isotropic	sample_conditions_1
3D HNCA	NaK_19	isotropic	sample_conditions_1
3D HNCACB	NaK_19	isotropic	sample_conditions_1

Software:

CCPNMR, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Avance 600 MHz
Bruker DMX 750 MHz
Varian INOVA 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks