BMRB Entry 26602

Name: Backbone 1H, 13C, 15N chemical shift assignments for T cell receptor D10 beta subunit
Published: 2015-07-14

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26602

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, 15N chemical shift assignments for T cell receptor D10 beta subunit

Deposition date:

2015-07-06

Original release date:

2015-07-14

Authors:

Mallis, Robert; Arthanari, Haribabu; Reinherz, Ellis; Wagner, Gerhard

Citation:

Citation: Mallis, Robert; Reinherz, Ellis; Wagner, Gerhard; Arthanari, Haribabu. "Backbone Resonance Assignment of N15, N30 and D10 T cell Receptor beta subunits" Biomol. NMR Assign. 10, 35-39 (2016).
PubMed: 26275917

Assembly members:

Assembly members:
D10_beta_TCR, polymer, 237 residues, Formula weight is not available

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet11d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
D10_beta_TCR: MAVTQSPRNKVAVTGGKVTL SCNQTNNHNNMYWYRQDTGH GLRLIHYSYGAGSTEKGDIP DGYKASRPSQENFSLILELA TPSQTSVYFCASGGQGRAEQ FFGPGTRLTVLEDLRNVTPP KVSLFEPSKAEIANKQKATL VCLARGFFPDHVELSWWVNG KEVHSGVSTDPQAYKESNYS YSLSSRLRVSATFWHNPRNH FRCQVQFHGLSEEDKWPEGS PKPVTQNISAEAWGRAD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	554
15N chemical shifts	192
1H chemical shifts	192

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	beta subunit	1

Entities:

Entity 1, beta subunit 237 residues - Formula weight is not available

1

MET

ALA

VAL

THR

GLN

SER

PRO

ARG

ASN

LYS

2

VAL

ALA

VAL

THR

GLY

LYS

VAL

THR

LEU

3

SER

CYS

ASN

GLN

THR

ASN

HIS

ASN

4

MET

TYR

TRP

TYR

ARG

GLN

ASP

THR

GLY

HIS

5

GLY

LEU

ARG

LEU

ILE

HIS

TYR

SER

TYR

GLY

6

ALA

GLY

SER

THR

GLU

LYS

GLY

ASP

ILE

PRO

7

ASP

GLY

TYR

LYS

ALA

SER

ARG

PRO

SER

GLN

8

GLU

ASN

PHE

SER

LEU

ILE

LEU

GLU

LEU

ALA

9

THR

PRO

SER

GLN

THR

SER

VAL

TYR

PHE

CYS

10

ALA

SER

GLY

GLN

GLY

ARG

ALA

GLU

GLN

11

PHE

GLY

PRO

GLY

THR

ARG

LEU

THR

VAL

12

LEU

GLU

ASP

LEU

ARG

ASN

VAL

THR

PRO

13

LYS

VAL

SER

LEU

PHE

GLU

PRO

SER

LYS

ALA

14

GLU

ILE

ALA

ASN

LYS

GLN

LYS

ALA

THR

LEU

15

VAL

CYS

LEU

ALA

ARG

GLY

PHE

PRO

ASP

16

HIS

VAL

GLU

LEU

SER

TRP

VAL

ASN

GLY

17

LYS

GLU

VAL

HIS

SER

GLY

VAL

SER

THR

ASP

18

PRO

GLN

ALA

TYR

LYS

GLU

SER

ASN

TYR

SER

19

TYR

SER

LEU

SER

ARG

LEU

ARG

VAL

SER

20

ALA

THR

PHE

TRP

HIS

ASN

PRO

ARG

ASN

HIS

21

PHE

ARG

CYS

GLN

VAL

GLN

PHE

HIS

GLY

LEU

22

SER

GLU

ASP

LYS

TRP

PRO

GLU

GLY

SER

23

PRO

LYS

PRO

VAL

THR

GLN

ASN

ILE

SER

ALA

24

GLU

ALA

TRP

GLY

ARG

ALA

ASP

Samples:

sample_1: D10 beta TCR, [U-13C; U-15N; U-2H], 0.4 mM; sodium chloride 150 mM; potassium phosphate 50 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 250 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.90, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance 750 MHz
Bruker Avance 600 MHz
Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks