BMRB Entry 26601

Name: Backbone 1H, 13C, 15N chemical shift assignments for T cell receptor N30 beta subunit
Published: 2015-07-14

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26601

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, 15N chemical shift assignments for T cell receptor N30 beta subunit

Deposition date:

2015-07-06

Original release date:

2015-07-14

Authors:

Mallis, Robert; Arthanari, Haribabu; Reinherz, Ellis; Wagner, Gerhard

Citation:

Citation: Mallis, Robert; Reinherz, Ellis; Wagner, Gerhard; Arthanari, Haribabu. "Backbone Resonance Assignment of N15, N30 and D10 T cell Receptor beta subunits" Biomol. NMR Assign. 10, 35-39 (2016).
PubMed: 26275917

Assembly members:

Assembly members:
N30_beta_TCR, polymer, 241 residues, Formula weight is not available

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet11d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
N30_beta_TCR: MEAGVTQSPRYAVLQEGQSV SFWCDPISGHDTLYWYQQPR DQGPQLLVYFRDEAVIDNSQ LPSDRFSAVRPKGTNSTLKI QSAKQGDTATYLCASSSGVG TEVFFGKGTRLTVVEDLRNV TPPKVSLFEPSKAEIANKQK ATLVCLARGFFPDHVELSWW VNGKEVHSGVSTDPQAYKES NYSYSLSSRLRVSATFWHNP RNHFRCQVQFHGLSEEDKWP EGSPKPVTQNISAEAWGRAD S

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	606
15N chemical shifts	207
1H chemical shifts	207

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	beta subunit	1

Entities:

Entity 1, beta subunit 241 residues - Formula weight is not available

1

MET

GLU

ALA

GLY

VAL

THR

GLN

SER

PRO

ARG

2

TYR

ALA

VAL

LEU

GLN

GLU

GLY

GLN

SER

VAL

3

SER

PHE

TRP

CYS

ASP

PRO

ILE

SER

GLY

HIS

4

ASP

THR

LEU

TYR

TRP

TYR

GLN

PRO

ARG

5

ASP

GLN

GLY

PRO

GLN

LEU

VAL

TYR

PHE

6

ARG

ASP

GLU

ALA

VAL

ILE

ASP

ASN

SER

GLN

7

LEU

PRO

SER

ASP

ARG

PHE

SER

ALA

VAL

ARG

8

PRO

LYS

GLY

THR

ASN

SER

THR

LEU

LYS

ILE

9

GLN

SER

ALA

LYS

GLN

GLY

ASP

THR

ALA

THR

10

TYR

LEU

CYS

ALA

SER

GLY

VAL

GLY

11

THR

GLU

VAL

PHE

GLY

LYS

GLY

THR

ARG

12

LEU

THR

VAL

GLU

ASP

LEU

ARG

ASN

VAL

13

THR

PRO

LYS

VAL

SER

LEU

PHE

GLU

PRO

14

SER

LYS

ALA

GLU

ILE

ALA

ASN

LYS

GLN

LYS

15

ALA

THR

LEU

VAL

CYS

LEU

ALA

ARG

GLY

PHE

16

PHE

PRO

ASP

HIS

VAL

GLU

LEU

SER

TRP

17

VAL

ASN

GLY

LYS

GLU

VAL

HIS

SER

GLY

VAL

18

SER

THR

ASP

PRO

GLN

ALA

TYR

LYS

GLU

SER

19

ASN

TYR

SER

TYR

SER

LEU

SER

ARG

LEU

20

ARG

VAL

SER

ALA

THR

PHE

TRP

HIS

ASN

PRO

21

ARG

ASN

HIS

PHE

ARG

CYS

GLN

VAL

GLN

PHE

22

HIS

GLY

LEU

SER

GLU

ASP

LYS

TRP

PRO

23

GLU

GLY

SER

PRO

LYS

PRO

VAL

THR

GLN

ASN

24

ILE

SER

ALA

GLU

ALA

TRP

GLY

ARG

ALA

ASP

25

SER

Samples:

sample_1: N30 beta TCR, [U-13C; U-15N; U-2H], 0.4 mM; sodium chloride 150 mM; sodium phosphate 50 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 250 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.90, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance 750 MHz
Bruker Avance 600 MHz
Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks