BMRB Entry 26600

Name: Backbone 1H, 13C, 15N chemical shift assignments for T cell receptor N15 beta subunit
Published: 2015-07-14

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26600

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, 15N chemical shift assignments for T cell receptor N15 beta subunit

Deposition date:

2015-07-06

Original release date:

2015-07-14

Authors:

Mallis, Robert; Arthanari, Haribabu; Reinherz, Ellis; Wagner, Gerhard

Citation:

Citation: Mallis, Robert; Reinherz, Ellis; Wagner, Gerhard; Arthanari, Haribabu. "Backbone Resonance Assignment of N15, N30 and D10 T cell Receptor beta subunits" Biomol. NMR Assign. 10, 35-39 (2016).
PubMed: 26275917

Assembly members:

Assembly members:
N15_beta_TCR, polymer, 239 residues, Formula weight is not available

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet11d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
N15_beta_TCR: DSGVVQSPRHIIKEKGGRSV LTCIPISGHSNVVWYQQTLG KELKFLIQHYEKVERDKGFL PSRFSVQQFDDYHSEMNMSA LELEDSAMYFCASSLRWGDE QYFGPGTRLTVLEDLRNVTP PKVSLFEPSKAEIANKQKAT LVCLARGFFPDHVELSWWVN GKEVHSGVSTDPQAYKESNY SYSLSSRLRVSATFWHNPRN HFRCQVQFHGLSEEDKWPEG SPKPVTQNISAEAWGRADS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	580
15N chemical shifts	198
1H chemical shifts	198

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	beta subunit	1

Entities:

Entity 1, beta subunit 239 residues - Formula weight is not available

1

ASP

SER

GLY

VAL

GLN

SER

PRO

ARG

HIS

2

ILE

LYS

GLU

LYS

GLY

ARG

SER

VAL

3

LEU

THR

CYS

ILE

PRO

ILE

SER

GLY

HIS

SER

4

ASN

VAL

TRP

TYR

GLN

THR

LEU

GLY

5

LYS

GLU

LEU

LYS

PHE

LEU

ILE

GLN

HIS

TYR

6

GLU

LYS

VAL

GLU

ARG

ASP

LYS

GLY

PHE

LEU

7

PRO

SER

ARG

PHE

SER

VAL

GLN

PHE

ASP

8

ASP

TYR

HIS

SER

GLU

MET

ASN

MET

SER

ALA

9

LEU

GLU

LEU

GLU

ASP

SER

ALA

MET

TYR

PHE

10

CYS

ALA

SER

LEU

ARG

TRP

GLY

ASP

GLU

11

GLN

TYR

PHE

GLY

PRO

GLY

THR

ARG

LEU

THR

12

VAL

LEU

GLU

ASP

LEU

ARG

ASN

VAL

THR

PRO

13

PRO

LYS

VAL

SER

LEU

PHE

GLU

PRO

SER

LYS

14

ALA

GLU

ILE

ALA

ASN

LYS

GLN

LYS

ALA

THR

15

LEU

VAL

CYS

LEU

ALA

ARG

GLY

PHE

PRO

16

ASP

HIS

VAL

GLU

LEU

SER

TRP

VAL

ASN

17

GLY

LYS

GLU

VAL

HIS

SER

GLY

VAL

SER

THR

18

ASP

PRO

GLN

ALA

TYR

LYS

GLU

SER

ASN

TYR

19

SER

TYR

SER

LEU

SER

ARG

LEU

ARG

VAL

20

SER

ALA

THR

PHE

TRP

HIS

ASN

PRO

ARG

ASN

21

HIS

PHE

ARG

CYS

GLN

VAL

GLN

PHE

HIS

GLY

22

LEU

SER

GLU

ASP

LYS

TRP

PRO

GLU

GLY

23

SER

PRO

LYS

PRO

VAL

THR

GLN

ASN

ILE

SER

24

ALA

GLU

ALA

TRP

GLY

ARG

ALA

ASP

SER

Samples:

sample_1: N15 beta TCR, [U-13C; U-15N; U-2H], 0.4 mM; sodium chloride 150 mM; sodium phosphate 50 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 250 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.90, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance 750 MHz
Bruker Avance 600 MHz
Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks