BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 26597

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the N-terminal active domain of the gyrase B subunit of Pseudomonas aeruginosa   PubMed: 26272827

Deposition date: 2015-07-05 Original release date: 2016-07-15

Authors: Li, Yan; ., Wong; ., .; ., .; ., .

Citation: Li, Yan; ., Wong; ., .; ., .; ., .; 26597, .; 8, 26597; Joseph, 9; Hill, Jeffrey; ., Keller; ., .. "NMR structural characterization of the N-terminal active domain of the gyrase B subunit from Pseudomonas aeruginosa and its complex with an inhibitor"  FEBS Lett. 589, 2683-2689 (2015).

Assembly members:
Pseudomonas_aeruginosa_DNA_gyrase_B_subunit, polymer, 229 residues, Formula weight is not available

Natural source:   Common Name: g-proteobacteria   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:   Production method: recombinant technology' 'Escherichia coli

Entity Sequences (FASTA):
Pseudomonas_aeruginosa_DNA_gyrase_B_subunit: MSENNTYDSSSIKVLKGLDA VRKRPGMYIGDTDDGTGLHH MVFEVVDNSIDEALAGYCSE ISITIHTDESITVRDNGRGI PVDIHKEEGVSAAEVIMTVL HAGGKFDVNTYKVSGGLHGV GVSVVNALSHELRLTIRRHN KVWEQVYHHGVPQFPLREVG ETDGSGTEVHFKPSPETFSN IHFSWDILAKRIRELSFLNS GVGILLRDERTGKEELFKYE GGLEHHHHH

Data sets:
Data typeCount
13C chemical shifts629
2181H chemical shifts

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA gyrase B1

Entities:

Entity 1, DNA gyrase B 229 residues - Formula weight is not available

1   MET.2659756TYR.265971112
2   LYS.265971718ASP.265972324
3   PRO.265972930ASP.265973536
4   GLY.265974142PHE.265974748
5   SER.265975354ALA.265975960
6   ILE.265976566THR.265977172
7   VAL.265977778GLY.265978384
8   HIS.265978990SER.265979596
9   MET.26597101102GLY.26597107108
10   ASN.26597113114GLY.26597119120
11   GLY.26597125126ALA.26597131132
12   ARG.26597137138HIS.26597143144
13   GLN.26597149150VAL.26597155156
14   ARG.26597161162ASP.26597167168
15   VAL.26597173174PRO.26597179180
16   ILE.26597185186ILE.26597191192
17   ARG.26597197198ASN.26597203204
18   LEU.26597209210THR.26597215216
19   PHE.26597221222LEU.26597227228
20   HIS

Samples:

sample_1: Pseudomonas aeruginosa DNA gyrase B subunit' '[U-13C; U-15N; U-2H]; .; . %; . 26597; . 6; ., 2, 26597 – 7 EDTA; 0.5 26597

sample_conditions_1: ionic strength: 80 mM; 6.5: . 26597; .: atm temperature; K: 26597

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
nosotropicnot availablenot available
not availablenot availablenot availablenot available
not availablenot available1not available
not availablenot available$sample_conditions_1not available
not availablesample_conditions_1not availablespectrometer_1
not availablenot availablenot availablenot available
not availablenot availablenot availablenot available

Software:

X-PLOR, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, 26597

NMR spectrometers:

  • Bruker Avance 700 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts