BMRB Entry 26593

Name: Human Obscurin Ig59
Published: 2016-07-11

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PDB ID: 2n56
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR26593
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Human Obscurin Ig59

Deposition date:

2015-06-25

Original release date:

2016-07-11

Authors:

Caldwell, Tracy; Wright, Nathan

Citation:

Citation: Caldwell, Tracy; Wright, Nathan. "Solution Structure of Ig59" .

Assembly members:

Assembly members:
Obscurin_Ig59, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet24A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Obscurin_Ig59: WRLEILELLKNAAVRAGAQA RFTCTLSEAVPVGEASWYIN GAAVQPDDSDWTVTADGSHQ ALLLRSAQPHHAGEVTFACR DAVASARLTVLGLPDGLEHH HHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	356
15N chemical shifts	94
1H chemical shifts	556

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Obscurin Ig59	1

Entities:

Entity 1, Obscurin Ig59 104 residues - Formula weight is not available

Residues 99-104 represent a non-native His-tag

1

TRP

ARG

LEU

GLU

ILE

LEU

GLU

LEU

LYS

2

ASN

ALA

VAL

ARG

ALA

GLY

ALA

GLN

ALA

3

ARG

PHE

THR

CYS

THR

LEU

SER

GLU

ALA

VAL

4

PRO

VAL

GLY

GLU

ALA

SER

TRP

TYR

ILE

ASN

5

GLY

ALA

VAL

GLN

PRO

ASP

SER

ASP

6

TRP

THR

VAL

THR

ALA

ASP

GLY

SER

HIS

GLN

7

ALA

LEU

ARG

SER

ALA

GLN

PRO

HIS

8

HIS

ALA

GLY

GLU

VAL

THR

PHE

ALA

CYS

ARG

9

ASP

ALA

VAL

ALA

SER

ALA

ARG

LEU

THR

VAL

10

LEU

GLY

LEU

PRO

ASP

GLY

LEU

GLU

HIS

11

HIS

Samples:

Ig59: Obscurin Ig59, [U-100% 13C; U-100% 15N], 1 mM; D2O, [U-100% 2H], 10%; TRIS 20 mM; sodium chloride 50 mM; sodium azide 350 uM; H2O 90%

sample_conditions_1: ionic strength: 100 mM; pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	Ig59	isotropic	sample_conditions_1
2D 1H-1H TOCSY	Ig59	isotropic	sample_conditions_1
3D CBCA(CO)NH	Ig59	isotropic	sample_conditions_1
3D C(CO)NH	Ig59	isotropic	sample_conditions_1
3D CBCA(CO)NH	Ig59	isotropic	sample_conditions_1
3D HNCO	Ig59	isotropic	sample_conditions_1
3D HN(CO)CA	Ig59	isotropic	sample_conditions_1
3D HNCACB	Ig59	isotropic	sample_conditions_1
3D H(CCO)NH	Ig59	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN v600 MHz, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks