BMRB Entry 26589

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26589

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for the yeast ADP/ATP carrier 3
Deposition date:
2015-06-17
Original release date:
2015-07-13
Authors:
Bruschweiler, Sven; Yang, Qin; Run, Changqing
Citation:

Citation: Bruschweiler, Sven; Yang, Qin; Run, Changqing; Chou, James. "Substrate Modulated Dynamics of the ADP/ATP Transporter Revealed by NMR Relaxation Dispersion"  Nat. Struct. Biol. ., .-..

Assembly members:

Assembly members:
yAAC3, polymer, 307 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
yAAC3: RGSDAKQQETNFAINFLMGG VSAAIAKTAASPIERVKILI QNQDEMIKQGTLDKKYSGIV DAFKRTAKQEGLISFWRGNT ANVIRYFPTQALNFAFKDKI KLMFGFKKEEGYGKWFAGNL ASGGAAGALSLLFVYSLDFA RTRLAADAKSSKKGGARQFN GLTDVYKKTLKSDGIAGLYR GFMPSVVGIVVYRGLYFGMF DSLKPLVLTGSLDGSFLASF LLGWVVTTGASTVSYPLDTV RRRMMMTSGQAVKYNGAIDA LKKIVASEGVGSLFKGAGAN ILRSVAGAGVISMYDQLQMI LFGKKFK

Data sets:
Data typeCount
13C chemical shifts669
15N chemical shifts256
1H chemical shifts256

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1yAAC31

Entities:

Entity 1, yAAC3 307 residues - Formula weight is not available

1   ARGGLYSERASPALALYSGLNGLNGLUTHR
2   ASNPHEALAILEASNPHELEUMETGLYGLY
3   VALSERALAALAILEALALYSTHRALAALA
4   SERPROILEGLUARGVALLYSILELEUILE
5   GLNASNGLNASPGLUMETILELYSGLNGLY
6   THRLEUASPLYSLYSTYRSERGLYILEVAL
7   ASPALAPHELYSARGTHRALALYSGLNGLU
8   GLYLEUILESERPHETRPARGGLYASNTHR
9   ALAASNVALILEARGTYRPHEPROTHRGLN
10   ALALEUASNPHEALAPHELYSASPLYSILE
11   LYSLEUMETPHEGLYPHELYSLYSGLUGLU
12   GLYTYRGLYLYSTRPPHEALAGLYASNLEU
13   ALASERGLYGLYALAALAGLYALALEUSER
14   LEULEUPHEVALTYRSERLEUASPPHEALA
15   ARGTHRARGLEUALAALAASPALALYSSER
16   SERLYSLYSGLYGLYALAARGGLNPHEASN
17   GLYLEUTHRASPVALTYRLYSLYSTHRLEU
18   LYSSERASPGLYILEALAGLYLEUTYRARG
19   GLYPHEMETPROSERVALVALGLYILEVAL
20   VALTYRARGGLYLEUTYRPHEGLYMETPHE
21   ASPSERLEULYSPROLEUVALLEUTHRGLY
22   SERLEUASPGLYSERPHELEUALASERPHE
23   LEULEUGLYTRPVALVALTHRTHRGLYALA
24   SERTHRVALSERTYRPROLEUASPTHRVAL
25   ARGARGARGMETMETMETTHRSERGLYGLN
26   ALAVALLYSTYRASNGLYALAILEASPALA
27   LEULYSLYSILEVALALASERGLUGLYVAL
28   GLYSERLEUPHELYSGLYALAGLYALAASN
29   ILELEUARGSERVALALAGLYALAGLYVAL
30   ILESERMETTYRASPGLNLEUGLNMETILE
31   LEUPHEGLYLYSLYSPHELYS

Samples:

sample_1: yAAC3, [U-100% 13C; U-100% 15N], 0.8 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.025 M; pH: 6; pressure: 1 atm; temperature: 303.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1

Software:

CCPN, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ GAA21635
EMBL CAA85031 CBK39159
GB AAA97485 AHY74567 AJP37166 AJP82061 AJP82451
REF NP_009642
SP P18238
TPG DAA07205
AlphaFold P18238

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks