BMRB Entry 26577

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26577
MolProbity Validation Chart

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for Light-adapted Cyanobacteriochrome NpR6012g4
Deposition date:
2015-05-11
Original release date:
2015-12-18
Authors:
Lim, Sunghyuk; Rockwell, Nathan; Martin, Shelley; Lagarias, J. Clark; Ames, James
Citation:

Citation: Lim, Sunghyuk; Rockwell, Nathan; Martin, Shelley; Lagarias, J. Clark; Ames, James. "1H, 13C, and 15N chemical shift assignments of cyanobacteriochrome NpR6012g4 in the green-absorbing photoproduct state"  Biomol. NMR Assign. 10, 157-161 (2016).
PubMed: 26537963

Assembly members:

Assembly members:
Cyanobacteriochrome_NpR6012g4, polymer, 180 residues, Formula weight is not available
entity_CYC, non-polymer, 588.694 Da.

Natural source:

Natural source:   Common Name: Nostoc Punctiforme   Taxonomy ID: 272131   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Nostoc Punctiforme

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cyanobacteriochrome_NpR6012g4: MGEKAVTKISNRIRQSSDVE EIFKTTTQEVRQLLRCDRVA VYRFNPNWTGEFVAESVAHT WVKLVGPDIKTVWEDTHLQE TQGGRYAQGENFVVNDIYQV GHSPCHIEILEQFEVKAYVI VPVFAGEQLWGLLAAYQNSG TRDWDESEVTLLARIGNQLG LALQQTEYLQQVQGQSAKPG

Data sets:
Data typeCount
13C chemical shifts643
15N chemical shifts167
1H chemical shifts1068

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cyanobacteriochrome NpR6012g41
2Phycocyanobilin2

Entities:

Entity 1, Cyanobacteriochrome NpR6012g4 180 residues - Formula weight is not available

1   METGLYGLULYSALAVALTHRLYSILESER
2   ASNARGILEARGGLNSERSERASPVALGLU
3   GLUILEPHELYSTHRTHRTHRGLNGLUVAL
4   ARGGLNLEULEUARGCYSASPARGVALALA
5   VALTYRARGPHEASNPROASNTRPTHRGLY
6   GLUPHEVALALAGLUSERVALALAHISTHR
7   TRPVALLYSLEUVALGLYPROASPILELYS
8   THRVALTRPGLUASPTHRHISLEUGLNGLU
9   THRGLNGLYGLYARGTYRALAGLNGLYGLU
10   ASNPHEVALVALASNASPILETYRGLNVAL
11   GLYHISSERPROCYSHISILEGLUILELEU
12   GLUGLNPHEGLUVALLYSALATYRVALILE
13   VALPROVALPHEALAGLYGLUGLNLEUTRP
14   GLYLEULEUALAALATYRGLNASNSERGLY
15   THRARGASPTRPASPGLUSERGLUVALTHR
16   LEULEUALAARGILEGLYASNGLNLEUGLY
17   LEUALALEUGLNGLNTHRGLUTYRLEUGLN
18   GLNVALGLNGLYGLNSERALALYSPROGLY

Entity 2, Phycocyanobilin - C33 H40 N4 O6 - 588.694 Da.

1   CYC

Samples:

sample_2: sodium phosphate 10 mM; Cyanobacteriochrome NpR6012g4, [U-99% 13C; U-99% 15N], 0.4 mM; D2O, [U-99% 2H], 7 % v/v; H2O 93 % v/v

sample_1: sodium phosphate 10 mM; Cyanobacteriochrome NpR6012g4, [U-99% 15N], 0.4 mM; D2O, [U-99% 2H], 7 % v/v; H2O 93 % v/v

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 301 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks