BMRB Entry 26575

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26575

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Title:
Gli3_1_90 assignment
Deposition date:
2015-05-08
Original release date:
2016-01-05
Authors:
Pierce, Wendy; Grace, Christy; Mittag, Tanja
Citation:

Citation: Pierce, Wendy; Grace, Christy; Lee, Jihun; Nourse, Amanda; Marzahn, Melissa; Watson, Edmond; High, Anthony; Peng, Junmin; Schulman, Brenda; Mittag, Tanja. "Multiple weak linear motifs enhance recruitment and processivity in SPOP-mediated substrate ubiquitination"  J. Mol. Biol. 428, 1256-1271 (2016).
PubMed: 26475525

Assembly members:

Assembly members:
Gli3_1_90, polymer, 90 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Gli3_1_90: MEAQSHSSTTTEKKKVENSI VKCSTRTDVSEKAVASSTTS NEDESPGQTYHRERRNAITM QPQNVQGLSKVSEEPSTSSD ERASLIKKEI

Data sets:
Data typeCount
13C chemical shifts256
15N chemical shifts86
1H chemical shifts86

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Gli3_1_901

Entities:

Entity 1, Gli3_1_90 90 residues - Formula weight is not available

1   METGLUALAGLNSERHISSERSERTHRTHR
2   THRGLULYSLYSLYSVALGLUASNSERILE
3   VALLYSCYSSERTHRARGTHRASPVALSER
4   GLULYSALAVALALASERSERTHRTHRSER
5   ASNGLUASPGLUSERPROGLYGLNTHRTYR
6   HISARGGLUARGARGASNALAILETHRMET
7   GLNPROGLNASNVALGLNGLYLEUSERLYS
8   VALSERGLUGLUPROSERTHRSERSERASP
9   GLUARGALASERLEUILELYSLYSGLUILE

Samples:

sample_1: Gli3_1_90, [U-100% 13C; U-100% 15N], 1.5 mM; potassium chloride 2.7 mM; sodium chloride 50.0 mM; potassium phosphate 1.76 mM; sodium phosphate 10.0 mM; DTT 5.0 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks